Amphotericin B-loaded nanoparticles for local treatment of cutaneous leishmaniasis

Date
2018-11-27
Authors
Abu Ammar, Aiman
Nasereddin, Abed
Ereqat, Suheir
Dan-Goor, Mary
Jaffe, Charles L.
Zussman, Eyal
Abdeen, Ziad
Journal Title
Journal ISSN
Volume Title
Publisher
Springer
Abstract
Cutaneous leishmaniasis (CL) is an infectious, parasitic disease caused by the protozoan Leishmania. Amphotericin B (AMB) is a macrolide polyene antibiotic presenting potent antifungal and antileishmanial activity, but due to poor water solubility at physiological pH, side effects, and toxicity, its therapeutic efficiency is limited. In the present study, poly(lactic-co-glycolic acid) (PLGA) nanoparticles (NPs) loaded with AMB were generated to reduce drug toxicity and facilitate localized delivery over a prolonged time. AMB NPs were characterized for particle size, zeta potential, polydispersity index, and degree of aggregation. In vitro assessments demonstrated its sustained activity against Leishmania major promastigotes and parasite-infected macrophages. A single intralesional administration to infected BALB/c mice revealed that AMB NPs were more effective than AMB deoxycholate in terms of reducing lesion area. Taken together, these findings suggest thatAMB NPs improve AMB delivery and can be used for local treatment of CL.
Description
Keywords
Amphotericin B, Nanoparticles, Sustained release, Cutaneous leishmaniasis, Topical therapy
Citation
TY - JOUR AU - Abu Ammar, Aiman AU - Nasereddin, Abedelmajeed AU - Ereqat, Suheir AU - Dan-Goor, Mary AU - Jaffe, Charles AU - Zussman, Eyal AU - Abdeen, Ziad PY - 2018/11/27 SP - T1 - Amphotericin B-loaded nanoparticles for local treatment of cutaneous leishmaniasis. Full-text: https://rdcu.be/bb6AP VL - DO - 10.1007/s13346-018-00603-0 JO - Drug Delivery and Translational Research ER -