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Phenolic and Flavonoids Analysis of Pomegranate Peel Extracts and their Antiniflammatory and Antioxidant Activities

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IJPQAVol10Issue1Article9.pdf (380.4Kb)
Date
2019-03-25
Author
K, Qabaha
F, Al-Rimawi
S, Nusseibeh
J, Abbadi
S, Abu-Lafi
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Abstract
An in-vitro evaluation of the anti-inflammatory and antioxidant activities of pomegranate peel extract from Palestine were investigated. In parallel, the total phenolic content (TPC) and the total flavonoids content (TFC) were measured. The antioxidant activities were determined spectrophotometrically by DPPH, FRAP, CUPRAC and the ABTS methods. The phenolic and flavonoid contents were separated and partially identified using HPLC and LC-MS. In-vitro inhibitory effect of the extract on production of Interlukin-6 (Il-6) and Tumor Necrosis Factor-α (TNF-α) by Lipopolysacaride (LPS)-induced polymorphonuclear Cells (PMNCs) was evaluated. Pomegranate peel extract was found to have strong antiinflamatory activity as revealed by the reduction in the levels of IL-6 and TNF-alfa. It was also found that it is rich in phenoloic and flavonoids that enhanced its reducing activity and free radical scavenging ability.
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https://dspace.alquds.edu/handle/20.500.12213/4974
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