عزل و وصف البكتيريا المحللة للفينول من نفايات معاصر الزيتون
Date
2018-01-10
Authors
احمد عبد الباسط جميل عبد القادر
Ahmad Abdelbaset Jamel Abdelkader
Journal Title
Journal ISSN
Volume Title
Publisher
AL-Quds University
جامعة القدس
جامعة القدس
Abstract
This study aimed to isolate of phenol degrading bacteria from olive mill wastes in
Palestine. The efficiency of phenol removal and factors affecting phenol degradation were
investigated. A bacterial strain (J20) was isolated from solid olive mill waste and identified as
Bacillus thuringiensis based on standard morphological, biochemical characteristics and
16SrRNA sequence analysis. The strain was able to grow in a phenol concentration of 700 mg/L
as the sole carbon and energy source. The culture conditions showed a significant impact on the
ability of these cells to remove phenol. This strain exhibited optimum phenol degradation
performance at pH 6.57 and 30 C
o
.
Under the optimized conditions, this strain could degrade 88.6% of phenol (700 mg/L) within 96
h when the initial cell density was OD600 0.2. However, the degradation efficiency could be
improved from about 88% to nearly 99% by increasing the cell density. Immobilization of J20
was carried out using 4% sodium alginate. Phenol degradation efficiency of the immobilized
cells of J20 was higher than that of the free cells, 100% versus 88.6% of 700 mg/L of phenol in
120 h, indicating the improved tolerance of the immobilized cells toward phenol toxicity.
The J20 was used in detoxifying crude OMWW, phenol levels were reduced by 61% compared
to untreated OMWW after five days of treatment. Hence, B. thuringiensis-J20 can be effectively
used for bioremediation of phenol-contaminated sites in Palestine. These findings may lead to
new biotechnological applications for the degradation of phenol, related to olive oil production.
Description
Keywords
الدراسات البيئية , Environmental Studies