Environmental Studies

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Browsing Environmental Studies by Subject "Environmental Science"

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    Fourier-Transform Infrared Spectroscopy as an aid for The determination of Cyanobacteria in surface water In Jericho.
    (AL-Quds University, 2007-06-09) نهى حجازي محمد حجازي; NUHA HIJAZI MOHD HIJAZI; معتز القطب; عامر مرعي
    The aim of this investigation is to study the possibility of using FTIR spectroscopy for monitoring water quality. Water samples from three sampling sites were collected; monthly samples around the year (January 2006 till January 2007) were collected from site I (Jericho Aquaculture pond) one sample from site II (Jericho agriculture pond). While one sample was collected from sampling site III which was Sulaiman pond. All of these samples have been screened by the microscope for any presented microorganisms. Microscope results showed that all water sample collected from site I and site II contain Oscillatoria (cyanobacteria), which was dominant all over the year in site I and in site II. This dominance is mainly due to high nutrient levels in Jericho aquaculture in addition to the environmental conditions that are suitable for this microorganism. In the water sample that was collected from site III no Oscillatoria was presented, but few types of invertebrates and other types of microorganisms. To check the validity of FTIR in determining the type and amount of these microorganisms all water samples have been checked by FTIR . Results of FTIR showed Oscillatoria fingerprint in all samples collected from site I and site II. A different fingerprint was obtained for sample from site III. A standard of Oscillatoria was prepared to compare results with, Oscillatoria standard spectrum was identical to all spectra of samples collected from site I and site II, but completely different fingerprints for site III. In the quantitative test: in order to study the optimization of FTIR in Quantitative analysis of microorganisms. The same water sample that was containing Oscillatoria was exposed to sunlight to enhance Oscillatoria growth for one week. Daily water sample with about 100ml volume of water was filtered. All dry samples collected on the filter paper were milled and prepared as Kbr disks for FTIR analysis. FTIR showed that when the quantity of Oscillatoria was increased (proliferation enhanced by continues exposure to sunlight) the absorbance was increased (Transmittance was decreased) which reflect the suitability of FTIR in quantitative determination of these microorganisms. Various porosity filter papers was used in another investigation the effect of the porosity of filter paper on the diversity of microorganism presented in water samples. Results showed that Oscillatoria fingerprints were clearly identified. The results of these investigations show that FTIR is very accurate in determination of water quality because it can determine type and amount of microorganism found in water, early determination of water quality will lead to sustainable critical water resources. A rapid inexpensive method for water quality determination like FTIR is strongly recommended.