Spectroscopic approach of the interaction study of Ceftriaxone and human serum albumin

Date
2013-12-10
Authors
Abu Teir, M. M.
Ghithan, J.
Abu-Taha, M. I.
Darwish, S. M.
Abu-hadid, M. M
Journal Title
Journal ISSN
Volume Title
Publisher
Academic Journals
Abstract
Under physiological conditions, interaction between ceftriaxone and human serum albumin was investigated by using fluorescence spectroscopy and ultra violet (UV) absorption spectrum. From spectral analysis, ceftriaxone showed a strong ability to quench the intrinsic fluorescence of human serum albumin (HSA) through a static quenching procedure. The binding constant (k) is estimated as K=1.02× 103 M-1 at 298 K. Fourier transform infrared spectroscopy (FT-IR) spectroscopy with Fourier self-deconvolution technique was used to determine the protein secondary structure and drug binding mechanisms. The observed spectral changes indicated the formation of H-bonding between ceftriaxone and HSA molecules at higher percentage for -helix than for the -sheets.
Description
Keywords
Ceftriaxone, amide I-III, binding mode, binding constant, protein secondary structure, Fourier transform IR, UV-spectroscopy, Flurosence spectroscopy
Citation