Development of polymorphic EST microsatellite markers for the sand fly, Phlebotomus papatasi (Diptera: Psychodidae) Hamarsheh, Omar Karakuş, Mehmet Azmi, Kifaya Jaouadi, Kaouther Yaghoobi-Ershadi, Mohammad Reza Krüger, Andreas Amro, Ahmad Kenawy, Mohamed Amin Dokhan, Mostafa Ramadhan Abdeen, Ziad McDowell, Mary Ann 2020-01-29T19:43:42Z 2020-01-29T19:43:42Z 2018-03-09
dc.description.abstract Background: Phlebotomus papatasi is a widely distributed sand fly species in different tropical and sub-tropical regions including the Middle East and North Africa. It is considered an important vector that transmits Leishmania major parasites, the causative agents of cutaneous leishmaniasis. The development of microsatellite markers for this sand fly vector is of high interest to understand its population structure and to monitor its geographic dispersal. Results: Fourteen polymorphic microsatellite markers were developed with simple di-, tri- and tetra-nucleotide repeats. The F statistics calculated for the 14 markers revealed high genetic diversity; expected heterozygosity (He) ranged from 0.407 to 0.767, while observed heterozygosity (Ho) was lower and ranged from 0.083 to 0.514. The number of alleles sampled fall in the range of 9–29. Three out of 14 markers deviated from Hardy-Weinberg expectations, no significant linkage disequilibrium was detected and high values for inbreeding coefficient (FIS) were likely due to inbreeding. Conclusions: The development of these functional microsatellites enable a high resolution of P. papatasi populations. It opens a path for researchers to perform multi locus-based population genetic structure analyses, and comparative mapping, a part of the efforts to uncover the population dynamics of this vector, which is an important global strategy for understanding the epidemiology and control of leishmaniasis. en_US
dc.description.sponsorship Acknowledgements Not applicable. Funding This work was supported by a grant from the Eck Institute for Global Health at the University of Notre Dame and travel grants awarded to Omar Hamarsheh from Fulbright Association and Zamallah Academic Development Programme. en_US
dc.identifier.issn 1756-3305
dc.language.iso en en_US
dc.publisher BMC en_US
dc.subject Phlebotomus papatasi en_US
dc.subject Leishmania major en_US
dc.subject Microsatellites en_US
dc.subject Population structure en_US
dc.title Development of polymorphic EST microsatellite markers for the sand fly, Phlebotomus papatasi (Diptera: Psychodidae) en_US
dc.type Article en_US
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