Anti-inflammatory activity of eucalyptus spp. and pistascia lentiscus leaf extracts
| dc.contributor.author | Qabaha, Khaled | |
| dc.contributor.author | Abu Ras, Sari | |
| dc.contributor.author | Abbadi, Jehad | |
| dc.contributor.author | Al-Rimawi, Fuad | |
| dc.date.accessioned | 2020-02-23T19:55:27Z | |
| dc.date.available | 2020-02-23T19:55:27Z | |
| dc.date.issued | 2016-08-04 | |
| dc.description.abstract | Background: Eucalyptus spp. and Pistascia lentiscus are among the Palestinian trees that are traditionally used in folkloric medicine in treating many diseases; leaves of which are thought to have anti-inflammatory, antibacterial and antioxidant effects. The goal of this study is to evaluate the in vitro inhibitory effect of Eucalyptus spp. and Pistascia lentiscus extracts on Lipopolysacaride (LPS)-induced Interlukin-6 (Il-6) and Tumor Necrosis Factor-α (TNF-α) by polymorphonuclear Cells (PMNCs). Materials and Methods: Polymorphonuclear cells were isolated from the whole blood using Histopaque (Ficol-1077) method and then cultured in an enriched Roswell Park Memorial Institute (RBMI) medium. Supernatants’ Interlukin-6 (IL-6) and Tumor Necrosis Factor (TNF-α) levels were determined 24 hour after LPS stimulation. HPLC was employed to determine the concentration of phenolic compounds in the extracts. The concentrations of TNF-α and IL-6 were compared using paired-samples t test. Results: Eucalyptus spp. and Pistascia lentiscus leaves extracts have shown significant reduction in the levels of both Il-6 and TNF-α Gallic acid; a strong anti-inflammatory agent was found to be the major phenolic compound in both leaf extracts. However, other antiinflammatory phenolic compounds were detected in Pitascia lentiscus extract including syringic acid and p-coumaric acid, while chlorogenic acid was detected in Eucalyptus spp. leaf extract. Conclusion: Reduction in the levels of Il-6 and TNF-α upon the effect of both Eucalyptus spp. and Pistascia lentiscus extract is an indication of their anti-inflammatory effects. Our results may also indicate that the observed anti-inflammatory effect of the above extracts may be due to the presence of gallic acid and other phenolic compounds. | en_US |
| dc.identifier.citation | TY - JOUR AU - Qabaha, Khaled AU - Ras, Sari AU - Abbadi, Jehad AU - Al-Rimawi, Fuad PY - 2016/08/12 SP - 1 EP - 6 T1 - Anti-inflammatory activity of Eucalyptus spp. and Pistascia lentiscus leaf extracts VL - 13 DO - 10.21010/ajtcam.v13i5.1 JO - African Journal of Traditional, Complementary, and Alternative Medicines ER - | en_US |
| dc.identifier.issn | 0189-6016 | |
| dc.identifier.uri | https://dspace.alquds.edu/handle/20.500.12213/5078 | |
| dc.language.iso | en | en_US |
| dc.subject | Anti-inflammatory | en_US |
| dc.subject | Eucalyptus spp | en_US |
| dc.subject | Pistascia lentiscus | en_US |
| dc.subject | HPLC | en_US |
| dc.subject | TNF-alpha | en_US |
| dc.subject | IL-6 | en_US |
| dc.title | Anti-inflammatory activity of eucalyptus spp. and pistascia lentiscus leaf extracts | en_US |
| dc.type | Article | en_US |