Anti-inflammatory activity of eucalyptus spp. and pistascia lentiscus leaf extracts
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Date
2016-08-04
Authors
Qabaha, Khaled
Abu Ras, Sari
Abbadi, Jehad
Al-Rimawi, Fuad
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Abstract
Background: Eucalyptus spp. and Pistascia lentiscus are among the Palestinian trees that are traditionally used in folkloric medicine in
treating many diseases; leaves of which are thought to have anti-inflammatory, antibacterial and antioxidant effects. The goal of this
study is to evaluate the in vitro inhibitory effect of Eucalyptus spp. and Pistascia lentiscus extracts on Lipopolysacaride (LPS)-induced
Interlukin-6 (Il-6) and Tumor Necrosis Factor-α (TNF-α) by polymorphonuclear Cells (PMNCs).
Materials and Methods: Polymorphonuclear cells were isolated from the whole blood using Histopaque (Ficol-1077) method and then
cultured in an enriched Roswell Park Memorial Institute (RBMI) medium. Supernatants’ Interlukin-6 (IL-6) and Tumor Necrosis Factor
(TNF-α) levels were determined 24 hour after LPS stimulation. HPLC was employed to determine the concentration of phenolic
compounds in the extracts. The concentrations of TNF-α and IL-6 were compared using paired-samples t test.
Results: Eucalyptus spp. and Pistascia lentiscus leaves extracts have shown significant reduction in the levels of both Il-6 and TNF-α
Gallic acid; a strong anti-inflammatory agent was found to be the major phenolic compound in both leaf extracts. However, other antiinflammatory
phenolic compounds were detected in Pitascia lentiscus extract including syringic acid and p-coumaric acid, while
chlorogenic acid was detected in Eucalyptus spp. leaf extract.
Conclusion: Reduction in the levels of Il-6 and TNF-α upon the effect of both Eucalyptus spp. and Pistascia lentiscus extract is an
indication of their anti-inflammatory effects. Our results may also indicate that the observed anti-inflammatory effect of the above
extracts may be due to the presence of gallic acid and other phenolic compounds.
Description
Keywords
Anti-inflammatory , Eucalyptus spp , Pistascia lentiscus , HPLC , TNF-alpha , IL-6
Citation
TY - JOUR AU - Qabaha, Khaled AU - Ras, Sari AU - Abbadi, Jehad AU - Al-Rimawi, Fuad PY - 2016/08/12 SP - 1 EP - 6 T1 - Anti-inflammatory activity of Eucalyptus spp. and Pistascia lentiscus leaf extracts VL - 13 DO - 10.21010/ajtcam.v13i5.1 JO - African Journal of Traditional, Complementary, and Alternative Medicines ER -