Development and Validation of an HPLC-UV Method for Determination of Eight Phenolic Compounds in Date Palms
Date
2015-05-03
Authors
Al-Rimawi, Fuad
Odeh, Imad
Journal Title
Journal ISSN
Volume Title
Publisher
AOAC International
Abstract
A simple, precise, accurate, and selective method
was developed and validated for determination of
eight phenolic compounds (gallic acid,
p-hydroxybenzoic acid, vanilic acid, caffeic acid,
syringic acid, p-coumaric acid, ferulic acid, and
sinapic acid) in date palms. Separation was achieved
on an RP C18 column using the mobile phase
methanol–water with 2% acetic acid (18 + 82, v/v).
This method was validated according to the
requirements for new methods, which include
accuracy, precision, selectivity, robustness, LOD,
LOQ, linearity, and range. The method demonstrated
good linearity over the range 1–1000 ppm of gallic
acid, p-hydroxybenzoic acid, vanilic acid, caffeic
acid, and syringic acid with r2 greater than 0.99, and
in the range of 3–1000 ppm for p–coumaric acid,
ferulic acid, and sinapic acid with r2 greater than
0.99. The recovery of the eight phenolic compounds
ranged from 97.1 to 102.2%. The method is selective
because adjacent peaks of phenolic compounds
were well separated with good resolution. The
degree of reproducibility of the results obtained as
a result of small deliberate variations in the method
parameters and by changing analytical operators
proved that the method is robust and rugged.