Antileishmanial Potential of Crude Plant Extracts Derived from Medicinal Plants in Palestine

Hamarsheh, Omar
Azmi, Kifaya
Amro, Ahmad
Schultheis, Martina
Abdeen, Ziad
Firdessa, Rebuma
Sawalha, Khaled
Al-Rimawi, Fuad
Yaghmour, Reem
Moll, Heidrun
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Herbal and traditional medicine is commonly and widely used in Palestine. There has been no ethno pharmacological study to document the usefulness of traditional or medicinal plants from Palestine against leishmaniasis, a spectrum of severe parasitic diseases that occur worldwide and is caused by protozoa of the genus Leishmania. The aim of the present study was to collect and analyze some of the traditionally used medicinal plants from Palestine against Leishmania major parasites that cause cutaneous leishmaniasis. Plant materials were collected during spring and summer of the year 2011, identified and the voucher numbers were kept at Al-Quds University Gardens (AQUG). The whole plant (except roots), flowers, fruits or seeds were collected, washed with distilled water, air dried in the shade for 20 days and then powdered in an electric grinder. For each plant species, alcoholic and dimethyl sulfoxide extracts were tested in vitro against L. major promastigotes and their antileishmanial activities were evaluated by Alamar Blue bioassay. Twenty plant species belonging to14 families were examined for their in vitro anti-parasitic effect against L. major. Among the total crude extracts tested; five were found to have various levels of activities (20%), some extracts having significant antileishmanial activity with IC50 values ranging from 8.83 to 100 μg/mL. The most active crude extracts were from the shoots of Artemisia inculta and Malva sylvestris with activity of 84.1%, IC50 = 8.8 μg/mL. And 90.1%, IC50 = 19.5 μg/mL respectively. The results demonstrate that the crude extracts of Artemisia inculta and Malva sylvestris showed promising antileishmanial activity, further and extensive studies should be carried out; particularly bio-guided fractionation to identify the active fraction and further chemical characterization of structure
Hamarsheh O, Azmi K, Amro A, Schultheis M, Abdeen Z, et al. (2017) Antileishmanial Potential of Crude Plant Extracts Derived from Medicinal Plants in Palestine. Ann Clin Cytol Pathol 3(4): 1065.