Bicarbonate In-Vitro Effect on Beta-Hematin Inhibition by Artemisia sieberi Aqueous Infusion Jaber, Suhair Abu-Lafi, Saleh Lutgen, Pierre Abu-Remeleh, Qassem Akkawi, Mutaz 2018-09-29T13:33:35Z 2018-09-29T13:33:35Z 2015-02-20
dc.description.abstract Malaria is still considered the most threatening disease in Africa. Plasmodium; the malaria parasite, forms during its intra-erythrocytic stage a pigment called hemozoin, which acts as a protection shield against oxygen radical-mediated stress that leads to parasite’s death. Many drugs targeting hemozoin formation such as chloroquine and amodiaquine, but recently strains of Plasmodium have gained resistance to such drugs. Artemisia sieberi stem and leaf water infusion extract compared with A. sieberi bicarbonate aqueous infusion were tested using a semi-quantitative in-vitro method based on the inhibition of ferriprotoporphyrin IX (FP) bio- mineralization developed by Deharo et al. to reveal the differences in antimalarial activity. Reversed phase preparative liquid chromatography coupled to Photo Diode Array (HPLC-PDA) detector was also used to explain this dissimilarity in antimalarial activity. We found that A. sieberi bicarbonate aqueous infusion inhibits the formation of β-hematin better than standard water infusion. The bicarbonate addition increases the extraction of more compounds as the chromatographic HPLC results revealed. Other Artemisia plants (A. vulgaris and A. herba alba) were also tested to explore any inhibition effects. en_US
dc.identifier.issn 2042-7158
dc.language.iso en_US en_US
dc.publisher David Publishing en_US
dc.subject Malaria en_US
dc.subject Plasmodium en_US
dc.subject Hemozoin en_US
dc.subject Artemisia sieberi en_US
dc.subject bicarbonate en_US
dc.subject drug resistance en_US
dc.title Bicarbonate In-Vitro Effect on Beta-Hematin Inhibition by Artemisia sieberi Aqueous Infusion en_US
dc.type Article en_US
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