Comparison of extraction yields, antioxidant, antimicrobial activity and concentration of main components of Olea europaea leaf samples at different seasons and of different areas in Palestine
Date
2018-08-15
Authors
Hanania, Michel
Abu Khairan, Rawan
Al-Aaraj, Amira
Kayali, Ibrahim
Journal Title
Journal ISSN
Volume Title
Publisher
Sphinx Knowledge House
Abstract
Olea europaea is widely cultivated tree for oil production in the Mediterranean
area. The benefits of olive leaves refer to their vital polyphenols components. In this study, the
optimum way for the extraction of olive leaves using Soxhlet extractor and various
concentrations of ethanol as solvent was examined. In order to find the effect of environmental
conditions on the extraction yield and chemical composition of olive leaves, phenolic
components of olive leaf extract (Oleuropein and Rutin) were analyzed area-wise and seasonwise
by a new and reliable RP-HPLC method. Extracts were screened for two
pharmacological effects, namely antioxidant and antimicrobial activities. Thus, olive leaves
were collected from three different areas in Palestine [south (Hebron), center (Beit Jala) and
north (Tulkarm)] at two maturation stages [June 2014 (season 1) and October 2014 (season
2)]. Results showed that 75% EtOH was the best extracting solvent and season 2 gave higher
yields of extraction. Beit Jala samples showed higher extracts than the two other areas with
higher concentrations of Oleuropein, while Rutin was not detectable. Antioxidant activity was
higher for untreated samples and samples of Hebron and Beit Jala had similar values. All
samples showed good antimicrobial activity against Gram positive bacteria, while no
inhibition was detected against Gram negative bacteria.
Description
Keywords
Olive leaf , Soxhlet extraction , Oleuropein , Rutin , antimicrobial activity
Citation
TY - JOUR AU - Hanania, Michel AU - Abukhairan, Rawan AU - Al-Aaraj, Amira AU - Kayali, Ibrahim PY - 2018/01/01 SP - 253 EP - 262 T1 - Comparison of extraction yields, antioxidant, antimicrobial activity and concentration of main components of Olea europaea leaf samples at different seasons and of different areas in Palestine VL - 11 DO - 10.20902/IJPTR.2018.11307 JO - International Journal of PharmTech Research ER -