Pure Isolates and Preparative HPLC Fractions or Crude Extract of Inula viscosa: Effect on β-hematin Inhibition in vitro

dc.contributor.author Abu-Lafi, Saleh A
dc.contributor.author Akkawi, Mutaz
dc.contributor.author Abu-Remeleh, Qassem
dc.contributor.author Jaber, Suhair
dc.contributor.author Qutob, Mutaz
dc.contributor.author Lutgen, Pierre
dc.date.accessioned 2020-01-29T19:51:38Z
dc.date.available 2020-01-29T19:51:38Z
dc.date.issued 2018-01-01
dc.description.abstract The antimalarial activity of wild Inula viscosa (I. viscosa) plant leaves was investigated. The efficacy of the whole crude extract versus preparative HPLC fractions versus pure isolates were compared by measuring their effect on β-hematin inhibition in-vitro. The preparative HPLC experiments consisted of reversed phase preparative column (22.2mm x 250mm, 10μm) and linear gradient of water, acetonitrile as the mobile phase. Injection volume was 1000μl and the wavelengths range were from 200-450 nm using photodiode array detector (PDA). While fractions (IV, V and VI) showed antimalarial potential in comparison to chloroquine positive control, the rest of the fractions did not show any significant inhibition to the β-hematin formation. The antimalarial results showed that whole crude exact of the plant works better than the preparative fractions or the pure isolates presumably due to synergistic effect. The chemical identity of some of the pure isolates was explored using UHPLC-ESi-MS. Moreover, I. viscosa extract powder stayed stable over several years, while many other products such as Artemisia annua extract or Artemisin Combined Therapy (ACT) drugs rapidly lost their efficiency under tropical storage conditions. en_US
dc.description.sponsorship None. en_US
dc.identifier.citation Abu-Lafi S, Akkawi M, Abu-Remeleh Q, Jaber S, Qutob M, et al. (2018) Pure Isolates and Preparative HPLC Fractions or Crude Extract of Inula viscosa: Effect on β-hematin Inhibition in vitro. Pharm Pharmacol Int J 6(1): 00145. DOI: 10.15406/ppij.2018.06.00145 en_US
dc.identifier.issn 2373-6367
dc.identifier.uri https://dspace.alquds.edu/handle/20.500.12213/5061
dc.language.iso en en_US
dc.subject Inula viscose en_US
dc.subject Malaria en_US
dc.subject Hemozoin en_US
dc.subject β-hematin en_US
dc.subject Preparative HPLC en_US
dc.subject LC-MS en_US
dc.title Pure Isolates and Preparative HPLC Fractions or Crude Extract of Inula viscosa: Effect on β-hematin Inhibition in vitro en_US
dc.type Article en_US
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