Extract of Purslane and Its Application
Date
2019-05-22
Authors
Maysaa Mahmoud Khalil Al-Halaseh
ميساء محمود خليل الهلسة
Journal Title
Journal ISSN
Volume Title
Publisher
Al-Quds University
Abstract
Portulacaoleracea L. (Portulacaceae) is a plant rich in benefits which contains high levels of linolinic acid and has been used in many countries to treat fevers and antibacterial healing. It is also used in wound healing, an analgesic and as a muscle relaxant.
The aims of this research are to get thermodynamic stability, improved solubilization and bioavailability for purslane extracts by using microemulsion that can be employed in diabetic drugs, and to investigate the effect of purslane on diabetes type2. Samples of purslane were collected from the local markets from Al-Eizariya, in the mid of April 2018. The samples were dried under shade for two weeks until it turned completely dry, then it was grinded and extracted by soxhlet. Following that, the antioxidant activity (AA) was studied using Ferric Ion Reducing Antioxidant Power Assay (FRAP) method. Total Phenolic Content (TPC) and Total Flavonoids Content (TFC) tests were also conducted on the extracts. It was noticed that the (TPC) and (TFC) of the seeds were higher than the (TPC) and (TFC) of leaves, which represented 243.0±5.0mg/g, 58.8±8.0mg/g, respectively. The antioxidant activity of FRAP was investigated on oil and on leaves; the best result was found among the leaves which represents 1.30268±0.1mg/g.
Then this research aimed at formulating biocompatible microemulsion that can be used in pharmaceutical drug. This was done by using purslane seed extract as oil, Tween 80 and Tween 20 as a non-ionic surfactant, water and leaf extracts as aqueous phase. After that, five microemulsion systems were selected; (A, B, C, D, E) to find the widest range of microemulsion. In every system at least one component was changed. System A and system B were selected as the basic methods of extracting, yet the same solvent was used. For systems C, D, E leaves extract was selected as aqueous phase. However, we changed the surfactant and method of extracting. This way the biggest range of microemulsion was achieved , because oil and leaves extract from the same plant mixed .
The antibacterial effect of purslane leaves and seeds was studied on bacillus bacteria and Pseudomonas bacteria. The result showed that the extract of the leaves has an antibacterial activity against Bacillus subtilis, Pseudomonas syringae. On the other hand, the seedextract also showed a slight activity against Bacillus subtilisonly.
Finally the effect of purslane on diabetic 2 was investigated through antiglycation test. The extract of leaves was examined by fluorescence, the result showed that the purslane gives inhibition against glycation, which is the main reason of diabetic diseases. That means it is
iv
effective on diabetic disease. We conclude from this research that Purslane has a potential antioxidant, antibacterial application and antiglycation effect.