Optimum Conditions for Oleuropein Extraction from Olive Leaves
Date
2014-10-05
Authors
Yateem, H.
Afaneh, I.
Al-Rimawi, F.
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
In this study the effect of extraction solvent (type, composition, pH, and temperature), and the extraction method
(maceration and soxhlet) on the amount of oleuropein extracted from olive leaves obtained from West Bank
/Palestine was investigated. It was found that pure solvents (100% water, 100% methanol, and 100% ethanol) are
not good solvents for oleuropein extraction, while mixtures of the solvents (methanol/water and ethanol/water)
gave higher oleuropein content. It was found that 80% ethanol give the highest oleuropein content followed by
20% acetonitrile. Temperature of extraction was found to have a significant effect on the oleuropein content
where higher temperature gave higher oleuropein content. It was found also that the amount of oleuropein
extracted decreases with increase in pH where highest amount was obtained at pH 3. Soxhlet extraction was
found to give higher oleuropein content compared to maceration method.
Description
Keywords
Oleuropein , extraction , olive leaves , maceration , soxhlet