Development and Validation of a Reversed-Phase HPLC Method for Determination of Elaidic Acid in Oils and Fats
Development and Validation of a Reversed-Phase HPLC Method for Determination of Elaidic Acid in Oils and Fats
Date
2015-11-03
Authors
Al-Rimawi, Fuad
Abadi, Jihad
Afaneh, Ibraheem
Journal Title
Journal ISSN
Volume Title
Publisher
Science Domain
Abstract
A simple, precise, accurate, and selective method with low limit of quantitation (LOQ) was
developed and validated for analysis of elaidic acid which is the predominant trans fatty acids in
partially hydrogenated vegetable oils. Separation was achieved on a reversed-phase C18 column,
using mobile phase consisting of acetonitrile/water (80:20, v/v) containing 0.1% acetic acid, and
using UV detection at 205 nm. This method was validated according to the requirements for new
methods, which include accuracy, precision, selectivity, robustness, limit of detection (LOD), LOQ,
linearity and range. The current method demonstrates good linearity over the range of 3-1000 mg
L-1 of elaidic acid with r2 greater than 0.999. The recovery of elaidic acid in oils and fats ranges from 94.5 to 98.7%. The method is selective where elaidic acid is good separated from oleic acid
and other components of fats and oils with good resolution. The method is also precise where the
RSD of the peak areas of replicate injections of elaidic acid solution is less than 1%. The degree of
reproducibility of the results obtained as a result of small deliberate variations in the method
parameters and by changing analytical operators has proven that the method is robust and rugged.
Description
Keywords
Elaidic acid detection, method development, oil, trans fatty acids, validation