Bacterial inhibition of Orobanche aegyptiaca and Orobanche cernuaradical elongation
Bacterial inhibition of Orobanche aegyptiaca and Orobanche cernuaradical elongation
Date
2010-02-05
Authors
Barghouthi, Sameer
Salman, Mazen
Journal Title
Journal ISSN
Volume Title
Publisher
Taylor & Francis Online
Abstract
The ability of different bacteria to inhibit Orobanche radical elongation was
examined. Seeds of Orobanche aegyptiaca Pers. and O. cernua Loefl. were induced
to germinate with the Growth Regulator GR24 in the presence or absence of test
bacterium. Radical lengths were estimated microscopically on a scale relative to
seed length (0 5 seed lengths) after 5 days of incubation at 258C. The results
indicated that Pseudomonas aeruginosa QUBC1, P. fluorescens QUBC3, Bacillus
atrophaeus QUBC16, and B. subtilis QUBC18 significantly inhibited radical
elongation (P50.01) of both O. aegyptiaca and O. cernua relative to control
radicals, whereas Microbacterium hydrocarbonoxydans QUBC11 and Ochrobactrum
anthropi QUBC13 showed less inhibitory effects. Other bacterial isolates had
no inhibitory effects. Bacterial isolates were identified using the universal method
in addition to morphological and biochemical features. The establishment of the
inhibitory effect of the most promising isolates, B. atrophaeus QUBC16 and
P. aeruginosa QUBC1 on radical elongation of both Orobanche spp. is a step
towards utilizing such bacteria as biocontrol agents against O. aegyptiaca,
O. cernua, and potentially other Orobanche species.
Description
Keywords
radical elongation, Orobanche, Pseudomonas, Bacillus, biocontrol, GR24