Show simple item record

dc.contributor.authorDarwish, Saqer M.
dc.contributor.authorAbu sharkh, Sawsan E.
dc.contributor.authorAbu Teir, Musa M.
dc.contributor.authorMakharza, Sami A.
dc.contributor.authorAbu-hadid, Mahmoud M.
dc.date.accessioned2018-09-10T18:27:31Z
dc.date.available2018-09-10T18:27:31Z
dc.date.issued2010-01-29
dc.identifier.issn0022-2860
dc.identifier.urihttps://dspace.alquds.edu/handle/20.500.12213/889
dc.description.abstractThe interaction between pentobarbital and human serum albumin has been investigated. The basic binding interaction was studied by UV-absorption and fluorescence spectroscopy. From spectral analysis pentobarbital showed a strong ability to quench the intrinsic fluorescence of HSA through a static quenching procedure. The binding constant (k) is estimated at 1.812 104 M 1 at 293 K. FT-IR spectroscopy with Fourier self-deconvolution technique was used to determine the protein secondary structure and drug binding mechanisms. The observed spectral changes of HSA–pentobarbital complex indicate a larger intensity decrease in the absorption band of a-helix relative to that of b-sheets. This variation in intensity is related indirectly to the formation of H-bonding in the complex molecules, which accounts for the different intrinsic propensities of a-helix and b-sheets.en_US
dc.description.sponsorshipThis work is supported by the German Research Foundation DFG Grant No. DR228/24-2.en_US
dc.language.isoen_USen_US
dc.publisherElsevieren_US
dc.subjectPentobarbitalen_US
dc.subjectHSAen_US
dc.subjectBinding constanten_US
dc.subjectProtein secondary structureen_US
dc.subjectFT-IR spectroscopyen_US
dc.titleSpectroscopic investigations of pentobarbital interaction with human serum albuminen_US
dc.typeArticleen_US


Files in this item

Thumbnail

This item appears in the following Collection(s)

  • AQU researchers publications [382]
    AQU researchers publications
  • Biophysics Research Laboratory [16]
    The biophysics lab has been established at Al-Quds University in 2005 with a generous fund from the DFG of the Federal Republic of Germany. The lab has supported several research students in the fields of physics and biology; several of whom are currently finishing their Doctorates degrees in Germany and the U.S. The research lab provides services to students and faculty members in the physics, biology, chemistry, and environmental science programs, in addition to an open cooperation with the college of medicine, pharmacy and the Nanotechnology center.

Show simple item record