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Spectroscopic investigations of pentobarbital interaction with human serum albumin

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RES_173_JOURNAL_1.pdf (839.0Kb)
Date
2010-01-29
Author
Darwish, Saqer M.
Abu sharkh, Sawsan E.
Abu Teir, Musa M.
Makharza, Sami A.
Abu-hadid, Mahmoud M.
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Abstract
The interaction between pentobarbital and human serum albumin has been investigated. The basic binding interaction was studied by UV-absorption and fluorescence spectroscopy. From spectral analysis pentobarbital showed a strong ability to quench the intrinsic fluorescence of HSA through a static quenching procedure. The binding constant (k) is estimated at 1.812 104 M 1 at 293 K. FT-IR spectroscopy with Fourier self-deconvolution technique was used to determine the protein secondary structure and drug binding mechanisms. The observed spectral changes of HSA–pentobarbital complex indicate a larger intensity decrease in the absorption band of a-helix relative to that of b-sheets. This variation in intensity is related indirectly to the formation of H-bonding in the complex molecules, which accounts for the different intrinsic propensities of a-helix and b-sheets.
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https://dspace.alquds.edu/handle/20.500.12213/889
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  • AQU researchers publications [335]
  • Biophysics Research Laboratory [16]

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