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dc.contributor.authorDarwish, Saqer M.
dc.contributor.authorAiaidah, Shurook Y.
dc.contributor.authorKhalid, Imtiaz M.
dc.contributor.authorAbuteir, Musa M.
dc.contributor.authorQawasmi, Lena
dc.identifier.citationDarwish, S.M., Aiaidah, S.Y., Khalid, I.M., Abuteir, M.M. and Qawasmi, L. (2015) Spectroscopic Investigations of β-Amyloid Interactions with Propofol and L-Arginine. Open Journal of Biophysics, 5, 50-67.
dc.description.abstractBeta amyloid (Aβ) aggregation has been characterized to be responsible for several amyloid diseases. Fourier transform infrared (FTIR) spectroscopy, fluorescence, and atomic force microscopy (AFM) are used to investigate induced changes in the secondary structure of Aβ upon thermal denaturation and interaction with propofol and L-arginine. Spectral analysis has revealed an effective static quenching for the intrinsic fluorescence of Aβ by propofol and l-arginine with binding constants of 2.81 × 102 M−1 for Aβ-propofol and 0.37 × 102 M−1 for Aβ-L-arginine. Fourier self-deconvolution (FSD) technique has been used to evaluate the relative intensity changes in the spectra of the component bands in the amide I and amide II regions at different ligand’s concentration in the protein complex. The analysis showed a decrease in the intensities of the parallel beta bands of propofol and L-arginine interactions with Aβ, accompanied with an increase in the antiparallel bands for the Aβ-propofol interaction and a decrease for the Aβ-l-arginine interaction. The relative increase in peaks’ intensities at 1694 cm−1 and 1531 cm−1 for the propofol interaction is linked to the formation of oligomers in the protein.en_US
dc.description.sponsorshipThis work is supported by the German Research Foundation DFG grant No. DR228/24-2.en_US
dc.publisherScientific Research Publishingen_US
dc.subjectFTIR Spectroscopyen_US
dc.subjectOligomeric Aβen_US
dc.subjectAlzheimer’s Diseaseen_US
dc.subjectAmyloid β-Peptideen_US
dc.subjectAntiparallel β-Sheeten_US
dc.titleSpectroscopic Investigations of β-Amyloid Interactions with Propofol and L-Arginineen_US

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  • Biophysics Research Laboratory [16]
    The biophysics lab has been established at Al-Quds University in 2005 with a generous fund from the DFG of the Federal Republic of Germany. The lab has supported several research students in the fields of physics and biology; several of whom are currently finishing their Doctorates degrees in Germany and the U.S. The research lab provides services to students and faculty members in the physics, biology, chemistry, and environmental science programs, in addition to an open cooperation with the college of medicine, pharmacy and the Nanotechnology center.

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