Show simple item record

dc.contributor.authorAfergan, Eyal
dc.contributor.authorNajajreh, Yousef
dc.contributor.authorGutman, Dikla
dc.contributor.authorEpstein, Hila
dc.contributor.authorElmalak, Omar
dc.contributor.authorGolomb, Gershon
dc.date.accessioned2018-09-05T11:32:17Z
dc.date.available2018-09-05T11:32:17Z
dc.date.issued2010-10-15
dc.identifier.citationAfergan E, Najajreh Y, Gutman D, Epstein H, Elmalak O, et al. (2010) 31P-NMR and Differential Scanning Calorimetry Studies for Determining Vesicle’s Drug Physical State and Fraction in Alendronate Liposomes. J Bioanal Biomed 2: 125-131. doi:10.4172/1948-593X.1000035en_US
dc.identifier.issn1948-593X
dc.identifier.urihttps://dspace.alquds.edu/handle/20.500.12213/847
dc.description.abstractBackground: A liposomal delivery system requires a complete understanding of the physicochemical characteristics of the drug– liposome system in order to predict their behavior and stability in-vitro and in-vivo. Objectives: Develop a rapid and simple experimental method to determine the fractions of the drug, alendronate (ALN), encapsulated and as a free form distributed in the liposomal suspension, and the physical state of the encapsulated drug. Methods: 31P-NMR measurements utilizing Ga+3 as a shifting reagent in comparison to HPLC determinations, theoretical calculations and differential scanning calorimetry (DSC) studies of various liposomal ALN formulations. Results: The 31P-NMR demonstrated that titrating liposomal ALN with increasing amounts of Ga+3 induced a signifi cant shift in the exterior fraction without changing the interior fraction. Quantitative determination of the encapsulated and non-encapsulated fractions of ALN has been achieved at Ga+3 concentrations of 3.2-25mM. The DSC study revealed that none of the formulation ingredients is in a solid phase. Conclusions: 31P-NMR was found to be sensitive enough to allow accurate differentiation of the distributed fractions of ALN, encapsulated and the non-encapsulated free form. Based on theoretical calculations and DSC analysis it can be concluded that ALN is dissolved in the aqueous core of the liposome.en_US
dc.description.sponsorshipThis work was supported in part by a grant from Biorest Ltd., Israel (GG). GG is affi liated with the David R. Bloom Center for Pharmacy at The Hebrew University of Jerusalem.en_US
dc.language.isoen_USen_US
dc.publisherOMICS Publishing Groupen_US
dc.subject31P-NMRen_US
dc.subjectLiposomesen_US
dc.subjectEncapsulationen_US
dc.subjectDifferential scanning calorimetry (DSC)en_US
dc.subjectAlendronateen_US
dc.subjectGallium nitrateen_US
dc.title31P-NMR and differential scanning calorimetry studies for determining vesicles drug physical state and fraction in alendronate liposomesen_US
dc.typeArticleen_US


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record