31P-NMR and differential scanning calorimetry studies for determining vesicles drug physical state and fraction in alendronate liposomes

Simple item page
dc.contributor.authorAfergan, Eyal
dc.contributor.authorNajajreh, Yousef
dc.contributor.authorGutman, Dikla
dc.contributor.authorEpstein, Hila
dc.contributor.authorElmalak, Omar
dc.contributor.authorGolomb, Gershon
dc.date.accessioned2018-09-05T11:32:17Z
dc.date.available2018-09-05T11:32:17Z
dc.date.issued2010-10-15
dc.description.abstractBackground: A liposomal delivery system requires a complete understanding of the physicochemical characteristics of the drug– liposome system in order to predict their behavior and stability in-vitro and in-vivo. Objectives: Develop a rapid and simple experimental method to determine the fractions of the drug, alendronate (ALN), encapsulated and as a free form distributed in the liposomal suspension, and the physical state of the encapsulated drug. Methods: 31P-NMR measurements utilizing Ga+3 as a shifting reagent in comparison to HPLC determinations, theoretical calculations and differential scanning calorimetry (DSC) studies of various liposomal ALN formulations. Results: The 31P-NMR demonstrated that titrating liposomal ALN with increasing amounts of Ga+3 induced a signifi cant shift in the exterior fraction without changing the interior fraction. Quantitative determination of the encapsulated and non-encapsulated fractions of ALN has been achieved at Ga+3 concentrations of 3.2-25mM. The DSC study revealed that none of the formulation ingredients is in a solid phase. Conclusions: 31P-NMR was found to be sensitive enough to allow accurate differentiation of the distributed fractions of ALN, encapsulated and the non-encapsulated free form. Based on theoretical calculations and DSC analysis it can be concluded that ALN is dissolved in the aqueous core of the liposome.en_US
dc.description.sponsorshipThis work was supported in part by a grant from Biorest Ltd., Israel (GG). GG is affi liated with the David R. Bloom Center for Pharmacy at The Hebrew University of Jerusalem.en_US
dc.identifier.citationAfergan E, Najajreh Y, Gutman D, Epstein H, Elmalak O, et al. (2010) 31P-NMR and Differential Scanning Calorimetry Studies for Determining Vesicle’s Drug Physical State and Fraction in Alendronate Liposomes. J Bioanal Biomed 2: 125-131. doi:10.4172/1948-593X.1000035en_US
dc.identifier.issn1948-593X
dc.identifier.urihttps://dspace.alquds.edu/handle/20.500.12213/847
dc.language.isoen_USen_US
dc.publisherOMICS Publishing Groupen_US
dc.subject31P-NMRen_US
dc.subjectLiposomesen_US
dc.subjectEncapsulationen_US
dc.subjectDifferential scanning calorimetry (DSC)en_US
dc.subjectAlendronateen_US
dc.subjectGallium nitrateen_US
dc.title31P-NMR and differential scanning calorimetry studies for determining vesicles drug physical state and fraction in alendronate liposomesen_US
dc.typeArticleen_US
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
RES_14_JOURNAL_paper_7.pdf
Size:
793.67 KB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
AQU researchers publications