31P-NMR and differential scanning calorimetry studies for determining vesicles drug physical state and fraction in alendronate liposomes

dc.contributor.author Afergan, Eyal
dc.contributor.author Najajreh, Yousef
dc.contributor.author Gutman, Dikla
dc.contributor.author Epstein, Hila
dc.contributor.author Elmalak, Omar
dc.contributor.author Golomb, Gershon
dc.date.accessioned 2018-09-05T11:32:17Z
dc.date.available 2018-09-05T11:32:17Z
dc.date.issued 2010-10-15
dc.description.abstract Background: A liposomal delivery system requires a complete understanding of the physicochemical characteristics of the drug– liposome system in order to predict their behavior and stability in-vitro and in-vivo. Objectives: Develop a rapid and simple experimental method to determine the fractions of the drug, alendronate (ALN), encapsulated and as a free form distributed in the liposomal suspension, and the physical state of the encapsulated drug. Methods: 31P-NMR measurements utilizing Ga+3 as a shifting reagent in comparison to HPLC determinations, theoretical calculations and differential scanning calorimetry (DSC) studies of various liposomal ALN formulations. Results: The 31P-NMR demonstrated that titrating liposomal ALN with increasing amounts of Ga+3 induced a signifi cant shift in the exterior fraction without changing the interior fraction. Quantitative determination of the encapsulated and non-encapsulated fractions of ALN has been achieved at Ga+3 concentrations of 3.2-25mM. The DSC study revealed that none of the formulation ingredients is in a solid phase. Conclusions: 31P-NMR was found to be sensitive enough to allow accurate differentiation of the distributed fractions of ALN, encapsulated and the non-encapsulated free form. Based on theoretical calculations and DSC analysis it can be concluded that ALN is dissolved in the aqueous core of the liposome. en_US
dc.description.sponsorship This work was supported in part by a grant from Biorest Ltd., Israel (GG). GG is affi liated with the David R. Bloom Center for Pharmacy at The Hebrew University of Jerusalem. en_US
dc.identifier.citation Afergan E, Najajreh Y, Gutman D, Epstein H, Elmalak O, et al. (2010) 31P-NMR and Differential Scanning Calorimetry Studies for Determining Vesicle’s Drug Physical State and Fraction in Alendronate Liposomes. J Bioanal Biomed 2: 125-131. doi:10.4172/1948-593X.1000035 en_US
dc.identifier.issn 1948-593X
dc.identifier.uri https://dspace.alquds.edu/handle/20.500.12213/847
dc.language.iso en_US en_US
dc.publisher OMICS Publishing Group en_US
dc.subject 31P-NMR en_US
dc.subject Liposomes en_US
dc.subject Encapsulation en_US
dc.subject Differential scanning calorimetry (DSC) en_US
dc.subject Alendronate en_US
dc.subject Gallium nitrate en_US
dc.title 31P-NMR and differential scanning calorimetry studies for determining vesicles drug physical state and fraction in alendronate liposomes en_US
dc.type Article en_US
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