Fourier Transform Infrared Spectroscopy Typing an Enterococcus sp.
Fourier Transform Infrared Spectroscopy Typing an Enterococcus sp.
Date
2014-10-05
Authors
Mohammad Abu Taha
Eideh, Hatem
Saed, Sameeh
Jaber, Hazem
Journal Title
Journal ISSN
Volume Title
Publisher
Scientific World
Abstract
Enterococcus species are one of the leading causes of nosocomial infections, which are difficult to
treat specially with the rise of its Vancomycin resistant. Studies of Enterococcus isolates are essential
for epidemiological investigation. Typing Enterococci is often based on the traditional phenotypic
as well as genotypic methods. In this study Fourier-transform infrared (FTIR) spectroscopy
is used as a novel phenotypic approach to the typing of Enterococci. FTIR spectroscopy results
compared to antibiotic susceptibility testing and PCR amplification of Vancomycin gene results;
the analysis showed that, 6 isolates were positive for Van gene (4 of VanA, 1 of VanB and 1 VanA
plus VanB). Three of VanA and VanA plus VanB were resistant to all antibiotic tested (Ampicillin,
Teicoplamin and Vancomycin) and VanB was found to be sensitive. FTIR spectroscopy (first derivatives)
divided the isolates into 8 groups. 3 groups of VanA (4 isolates), one of VanB (one isolate),
one of VanA plus VanB (one isolate) and the other 13 Enterococcus isolates were divided into 3
clusters. The study demonstrated that FTIR spectroscopy has good discriminative capacity and
high reproducibility as compared to other techniques.
Description
Keywords
Enterococci, Antibiotic Susceptibility Testing, FTIR Spectroscopy, PCR, VRE Typing