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dc.contributor.authorKilliny, Nabil
dc.contributor.authorGonzalez-Blanco, Pedro
dc.contributor.authorSantos-Ortega, Yulica
dc.contributor.authorAl-Rimawi, Fuad
dc.contributor.authorLevy, Amit
dc.contributor.authorHijaz, Faraj
dc.contributor.authorAlbrecht, Ute
dc.contributor.authorBatuman, Ozgur
dc.date.accessioned2020-08-08T13:09:05Z
dc.date.available2020-08-08T13:09:05Z
dc.date.issued2019-12-12
dc.identifier.issn2079-6382
dc.identifier.urihttps://dspace.alquds.edu/handle/20.500.12213/5737
dc.description.abstractHuánglóngbìng (HLB), citrus greening, is one of the most destructive diseases of citrusplants worldwide. In North America, HLB is caused by the phloem-limited bacteriumCandidatusLiberibacter asiaticus and is transmitted by the Asian citrus psyllid,Diaphorina citri. No cure exists atpresent, and the use of antibiotics for the control of HLB has gained interest due to the significantlosses to the citrus industry. Because of unsatisfactory results when using foliar applications ofantibiotics, concerns were raised regarding the uptake and translocation of these materials within trees.We, therefore, investigated a method that allows us to study the movement of antibiotic materialsin citrus plants. Herein, we utilized a fluorescence-labeled penicillin, BOCILLIN™FL-Penicillin(FL-penicillin), to study the uptake and translocation of penicillin in citrus plants. FL-penicillin wasapplied by puncture to the stem of young citrus seedlings and was traced by using fluorescencemicroscopy. After application, we detected FL-penicillin in the leaves and in the stem xylem andphloem tissues above and below the application site in both intact and partially bark-girdled citrusseedlings, indicating that it is easily taken up and transported through the plant vascular system.In addition, we detected FL-penicillin in the gut ofD. citri, which were allowed to feed on thetreated plants, suggesting translocation of this molecule into the vascular tissue. We propose thatthe use of fluorescent-labeled molecules could be an effective tool for understanding the uptake andtranslocation of antibiotics and other macromolecules in plants and insects.en_US
dc.description.sponsorshipFunding:This research was funded by the National Institute of Food and Agriculture, grant number2019-70016-29096.Acknowledgments:We thank our lab members for helpful discussion and technical assistance. This work wasgenerously funded by the USDA NIFA-SCRI award number 2019-70016-29096.en_US
dc.language.isoenen_US
dc.publisherMDPIen_US
dc.subjectBOCILLIN™FL-Penicillinen_US
dc.subjecttranslocation;en_US
dc.subjectuptakeen_US
dc.subjectcitrusen_US
dc.subjectHuanglongbing (HLB)en_US
dc.subjectxylemen_US
dc.subjectphloemen_US
dc.titleTracing Penicillin Movement in Citrus Plants Using Fluorescence-Labeled Penicillinen_US
dc.typeArticleen_US


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