Tracing Penicillin Movement in Citrus Plants Using Fluorescence-Labeled Penicillin

dc.contributor.author Killiny, Nabil
dc.contributor.author Gonzalez-Blanco, Pedro
dc.contributor.author Santos-Ortega, Yulica
dc.contributor.author Al-Rimawi, Fuad
dc.contributor.author Levy, Amit
dc.contributor.author Hijaz, Faraj
dc.contributor.author Albrecht, Ute
dc.contributor.author Batuman, Ozgur
dc.date.accessioned 2020-08-08T13:09:05Z
dc.date.available 2020-08-08T13:09:05Z
dc.date.issued 2019-12-12
dc.description.abstract Huánglóngbìng (HLB), citrus greening, is one of the most destructive diseases of citrusplants worldwide. In North America, HLB is caused by the phloem-limited bacteriumCandidatusLiberibacter asiaticus and is transmitted by the Asian citrus psyllid,Diaphorina citri. No cure exists atpresent, and the use of antibiotics for the control of HLB has gained interest due to the significantlosses to the citrus industry. Because of unsatisfactory results when using foliar applications ofantibiotics, concerns were raised regarding the uptake and translocation of these materials within trees.We, therefore, investigated a method that allows us to study the movement of antibiotic materialsin citrus plants. Herein, we utilized a fluorescence-labeled penicillin, BOCILLIN™FL-Penicillin(FL-penicillin), to study the uptake and translocation of penicillin in citrus plants. FL-penicillin wasapplied by puncture to the stem of young citrus seedlings and was traced by using fluorescencemicroscopy. After application, we detected FL-penicillin in the leaves and in the stem xylem andphloem tissues above and below the application site in both intact and partially bark-girdled citrusseedlings, indicating that it is easily taken up and transported through the plant vascular system.In addition, we detected FL-penicillin in the gut ofD. citri, which were allowed to feed on thetreated plants, suggesting translocation of this molecule into the vascular tissue. We propose thatthe use of fluorescent-labeled molecules could be an effective tool for understanding the uptake andtranslocation of antibiotics and other macromolecules in plants and insects. en_US
dc.description.sponsorship Funding:This research was funded by the National Institute of Food and Agriculture, grant number2019-70016-29096.Acknowledgments:We thank our lab members for helpful discussion and technical assistance. This work wasgenerously funded by the USDA NIFA-SCRI award number 2019-70016-29096. en_US
dc.identifier.issn 2079-6382
dc.identifier.uri https://dspace.alquds.edu/handle/20.500.12213/5737
dc.language.iso en en_US
dc.publisher MDPI en_US
dc.subject BOCILLIN™FL-Penicillin en_US
dc.subject translocation; en_US
dc.subject uptake en_US
dc.subject citrus en_US
dc.subject Huanglongbing (HLB) en_US
dc.subject xylem en_US
dc.subject phloem en_US
dc.title Tracing Penicillin Movement in Citrus Plants Using Fluorescence-Labeled Penicillin en_US
dc.type Article en_US
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