A hplc-uv method for deteermination of three pesticides in water
A hplc-uv method for deteermination of three pesticides in water
Files
Date
2014-05-06
Authors
Al-Rimawi, Fuad
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
HPLC method is developed and validated for determination of three pesticides (abamectin, imidacloprid,
and -cyfluthrin) in water. These pesticides are used widely in agriculture for crops protection, and may be
leached to the groundwater. Reversed-phase method with C18 column (5 μm, 250mm × 4.6 mm inner
diameter) using a mobile phase consisting of acetonitrile/water (v:v = 4:1) at a flow rate of 1.5 mL/min and
UV detection at 220 nm was used. This method is validated according to new methods which include
accuracy, precision, linearity and range, limit of detection and limit of quantitation. The current method
exhibits good linearity over the range of 1-1000 ppb for abamectin, 0.5-1000 ppb for imidacloprid, and
0.4-1000 ppb for ß-cyfluthrin with r2 greater than 0.990. The percentage recovery of the method at three
concentration levels (5, 100, and 1000 ppb) is within 97.6 to 101.5% for the three pesticides. Relative
standard deviation of the area of six replicate injections of each pesticide at three concentration levels (5.0,
100.0, and 1000.0 ppb) was found to be less than 1% which reflect the precision of the method. Limit of
quantitation of the three pesticides using this method is low (1.0, 0.5, and 0.4 ppb) for abamectin,
imidacloprid, and -cyfluthrin, respectively which enables the determination of these three pesticides in
water at low concentration levels.
Description
Keywords
Abamectin, Imidacloprid, β-cyfluthrin, pesticides, HPLC-UV, validation