Investigating the impacts of various solvents fractions of Bupleurum lancifolium on the antimicrobial and antioxidant potentials
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Date
2017-11-20
Authors
Jaradat, Nidal
Al-Masri, Motasem
Al-Rimawi, Fuad
Zaid, Abdel Naser
Saboba, Malik Mohammad
Hussein, Fatima
Aker, Amanda
Qasem, Dalal
Hejazi, Saja
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Abstract
In the last thirty years, interests in searching for plants with potential antimicrobial
and antioxidant activities have been increased due to their probable health benefits.
This study aimed to investigate the antimicrobial and antioxidant effects of various solvents
fractions of Bupleurum lancifolium. Methods: The antioxidant activities of four fractions of Bupleurum
lancifolium were assessed by 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging assay,
while the antimicrobial activity was assessed by broth microdilution method. The antimicrobial
activity of four plant fractions was examined against Staphylococcus aureus, Escherichia
coli, Pseudomonas aeruginosa, Shigella sonnie and Enterococcus faecium American Type
Culture Collection reference strains. Results: The fractionation extraction method succeeds
in the assessment of the antibacterial and antioxidant activity of B. lancifolium plant. Hence
the methanolic fraction has antioxidant potential with 33.03% of inhibition according to the
Trolox antioxidant standard molecule, while n-hexane and methanol fractions showed powerful
antibacterial activity against E. faecium and S. sonnie strains with Minimum Inhibitory Concentration
(MIC)1.5625 mg/ml for both fractions. Conclusion: All plant fractions have potent
antioxidant and antibacterial activities. Further investigations, i.e. isolation, identification, and
clinical assessment, of the active compound(s) are needed for the possible formulation of new
therapeutic alternatives.
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Keywords
Bupleurum lancifolium , Antibacterial , Antioxidant , Palestine