Development and Validation of a Stability-Indicating HPLC Method for the Simultaneous Determination of Florfenicol and Flunixin Meglumine Combination in an Injectable Solution
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Date
2017-07-11
Authors
Batrawi, Nidal
Naseef, Hani
Al-Rimawi, Fuad
Journal Title
Journal ISSN
Volume Title
Publisher
Hindawi
Abstract
Thecombination of the powerful antimicrobial agent florfenicol and the nonsteroidal anti-inflammatory flunixin meglumine is used
for the treatment of bovine respiratory disease (BRD) and control of BRD-associated pyrexia, in beef and nonlactating dairy cattle.
This study describes the development and validation of an HPLC-UVmethod for the simultaneous determination of florfenicol and
flunixin, in an injectable preparation with a mixture of excipients.The proposed RP-HPLC method was developed by a reversed
phase- (RP-) C18e (250mm× 4.6 mm, 5 𝜇m) column at roomtemperature, with an isocraticmobile phase of acetonitrile and water
mixture, and pH was adjusted to 2.8 using diluted phosphoric acid, a flow rate of 1.0 mL/min, and ultraviolet detection at 268 nm.
The stability-indicating method was developed by exposing the drugs to stress conditions of acid and base hydrolysis, oxidation,
photodegradation, and thermal degradation; the obtained degraded products were successfully separated from the APIs. This
method was validated in accordance with FDA and ICH guidelines and showed excellent linearity, accuracy, precision, specificity,
robustness, LOD, LOQ, and system suitability results within the acceptance criteria.