Biochemistry & Molecular Biology الكيمياء الحيوية والأحياء الجزيئية

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Browsing Biochemistry & Molecular Biology الكيمياء الحيوية والأحياء الجزيئية by Author "Bushra Abdel Qader Tahboub Al-Amawi"

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    Genetic Analysis and Population Structure of Phlebotomus sergenti Sandflies; Vectors of Leishmania tropica in Palestine
    (Al-Quds University, 2024-05-19) Bushra Abdel Qader Tahboub Al-Amawi; بشرى عبدالقادر طهبوب الأموي
    Phlebotomus (Ph.) sergenti is a widespread sandfly, it is considered a vector of leishmania tropica in Palestine and other countries; it is the cause of cutaneous leishmaniasis. This study aimed to investigate and analyze the phylogenetic and phylogeographic structure of Ph. sergenti among separated populations in the West Bank; Palestine. Population analysis of these sandflies is necessary for developing models that may provide better understanding of their current geographic distribution. The genetic population structure analysis of fifty Ph. sergenti sandflies were collected from West Bank, Palestine. This study inspected the genetic differentiation between Ph. sergenti populations using a 442 bp fragment of Cyt. b mtDNA gene. Maximum parsimony tree and median joining network were constructed depending on the identified haplotypes. Additionally, one hundred Ph. sergenti sequences previously deposited in the GenBank from ten countries (Iran, Pakistan, Afghanistan, Turkey, Cyprus, Greece, Syria, Lebanon, Morocco and Spain), were included in the study. Nine haplotypes were obtained from the Palestinian populations; six of them were private haplotypes, suggesting the presence of genetic isolation. The global analysis exhibited forty-five global haplotypes and grouped according to their geographical location. Pairwise FST values ranged between 0.58533 and 0.89288, indicating a high genetic differentiation and low genetic flow. Population genetic analysis of Ph. sergenti is considered crucial and may be needed to design an appropriate insecticide spraying programs and for sandfly control models, to restrict the transmission of leishmania parasites in the endemic areas of cutaneous leishmaniasis.