Medical Laboratory science علوم المختبرات الطبية
Permanent URI for this collection
Browse
Browsing Medical Laboratory science علوم المختبرات الطبية by Author "Ahmad Ziyad Khalil Abdelkader"
Now showing 1 - 1 of 1
Results Per Page
Sort Options
- Item“Evaluation of antibody titers and serotypes in COVID-19 vaccinated human being”(Al-Quds University, 2025-01-04) Ahmad Ziyad Khalil Abdelkader; أحمد زياد خليل عبد القادرIntroduction: SARS-CoV-2 is a novel coronavirus that emerged in 2019 and is now classified in the genus Coronavirus with closely related SARS-CoV. Due to the high pathogenicity of this virus in humans, It is classified as biosafety level 3 (BSL-3) pathogen, which makes manipulating it relatively difficult due to its infectious nature that killed high number of people. Methods: To assessment immune system and antibodies titer the serum samples collected from 85 hospitalized vaccinated patients against COVID-19 disease or were previously infected patients and from normal non vaccinated negative donors. To evaluation of antibody titers an Enzyme-linked immunosorbent assay (ELISA) technology performed in laboratories, and evaluation the virus infection, and proteins M and S spike proteins of SARS-CoV-2 evaluated and determined for detection of spikebinding neutralizing antibody titers in sera from hospitalized COVID-19 vaccinated patients by using commercial enzyme and ELISA technology. Furthermore, to measure SARS-CoV-2 neutralizing antibodies under BSL conditions, an ELISA for SARS-CoV-2 was developed. Recording the results of laboratory analyzes and saving them in a database using a computer. Result: An analysis and assessment the antibodies titer was conducted and obtain results about the Corona virus, its spread and its effect on people, where the immunological and microbiological results of the people were analyzed and then enter this data as input into the laboratory database, and it was found from this study that there is a clear discrepancy between the data of vaccinated and healthy people, as it appeared that the vaccinated show higher titer than unvaccinated normal individuals. In conclusion, this study aimed to evaluate the long-term effectiveness of COVID-19 vaccination in Palestine people by analyzing they response to the vaccine after two years, based on anti-SARS-CoV-2 protein S and M antibody titer levels. This study demonstrates the importance of knowing the impact of the Corona virus on people, efficacy of vaccines and presence of neutralizing antibodies against Covid19. To evaluate an immune system throw examination by assessment antibody titer and serotypes specially using high specificity and sensitivity ELISA technology to COVID-19 system, which easily can be implemented and performed within a biosafety level laboratory (BSL) for evaluating the occurrence of neutralizing antibodies against SARS-CoV-2 and assessment of both S and M antigens to control and limit the transmission of the COVID 19 in order to find an appropriate treatment for this virus and improve new vaccination in Palestine