Development and Validation of a Simple Reversed‑Phase HPLC‑UV Method for Determination of Malondialdehyde in Olive Oil
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Date
2015-06-06
Authors
Al‑Rimawi, Fuad
Journal Title
Journal ISSN
Volume Title
Publisher
Springer
Abstract
A simple, precise, accurate and selective
method was developed and validated for determination
of malondialdehyde (MDA) in olive oil. Separation was
achieved on a reversed-phase C8 column using a mobile
phase consisting of methanol/0.8 % phosphoric acid
(10:90, v/v), at a flow rate of 1.0 ml/min and UV detection
at 220 nm. This method was validated according to
the requirements for new methods, which include accuracy,
precision, selectivity, robustness, a limit of detection,
limit of quantitation (LOQ), linearity and range. The current
method demonstrated good linearity over the range
of 0.5–1000 ppm of MDA with r2 greater than 0.999. The
recovery of MDA in olive oil ranged from 97.1 to 99.1 %.
The method was selective where MDA was distinctly separated
from other compounds of the oil with good resolution.
The method was also precise where the RSD of the
peak areas of replicate injections of MDA standard solution
were less than 1 %. The degree of reproducibility of
the results obtained as a result of small deliberate variations
in the method parameters and by changing the analytical
operators proved that the method is robust and rugged. The
low LOQ of MDA (0.5 ppm) using this method enables
quantitation of MDA at low concentration.
Description
Keywords
Malondialdehyde , Olive oil , Validation , Method development , HPLC , Oxidation
Citation
Development and Validation of a Simple Reversed‑Phase HPLC‑UV Method for Determination of Malondialdehyde in Olive Oil