Development and validation of a simple reversed phase HPLC-UV method for determination of oleuropein in olive leaves
Date
2014-09-04
Authors
Al-Rimawi, Fuad
Journal Title
Journal ISSN
Volume Title
Publisher
Elsevier
Abstract
A simple, precise, accurate, and selective method is developed and validated for the
determination of oleuropein, which is the main phenolic compound in olive leaves. Separation
was achieved on a reversed-phase C18 column (5 mm, 150 4.6 mm inner diameter)
using a mobile phase consisting of acetonitrile/phosphate buffer pH 3.0 (20:80, v/v), at a flow
rate of 1.0 mL/minute and UV detection at 280 nm. This method is validated according to the
requirements for new methods, which include accuracy, precision, selectivity, robustness,
limit of detection, limit of quantitation, linearity, and range. The current method demonstrates
good linearity over the range of 3e1000 ppm of oleuropein, with r2 > 0.999. The recovery
of oleuropein in olive leaves ranges from 97.7% to 101.1%. The method is selective, in
that oleuropein is well separated from other compounds of olive leaves with good resolution.
The method is also precisedthe relative standard deviation of the peak areas of
replicate injections of oleuropein standard solution is <1%. The degree of reproducibility of
the results obtained as a result of small deliberate variations in the method parameters and
by changing analytical operators has proven that the method is robust and rugged. The low
limit of detection and limit of quantitation of oleuropein when using this method enable the
detection and quantitation of oleuropein at low concentrations.
Description
Keywords
HPLC , Method development , Oleuropein , Olive leaves , Validation
Citation
Al-Rimawi F, Development and validation of a simple reversed-phase HPLC-UV method for determination of oleuropein in olive leaves, Journal of Food and Drug Analysis (2013), http://dx.doi.org/10.1016/ j.jfda.2013.10.002