Pomegranate peel (Punica granatum L.) versusـ Juice: Properties and Benefits
Date
2022-08-12
Authors
Eman Hasan Ismail Amar
ايمان حسن إسماعيل اعمر
Journal Title
Journal ISSN
Volume Title
Publisher
Al-Quds University
Abstract
The presence of antioxidants and polyphenols in pomegranate peels and juice has been linked to several medical benefits. In the juice, you'll find vitamins and minerals. It is possible to understand the pomegranate's features and benefits, which are beneficial for treating a variety of disorders, by using various extracts (alcoholic extract and ethyl acetate). To develop treatments for diverse ailments, this study is crucial.
In this study, the pomegranate peel was extracted using the Soxhlet extraction apparatus, which was able to essentially remove all of the active compounds from this plant.
The Folin-Ciocalteu test was used to measure the total phenolic content (TPC) of pomegranate peel extracts and juice. All tests were performed using a UV-Visible spectrophotometer. Pomegranate juice and peel were shown to have antioxidant capabilities and high phenolic content. While the total phenolic content (TPC) of pomegranate peel extracted with ethanol 99% produced a result of (78.81mg/g) higher than that of pomegranate peel extracted with, ethanol 35% which is (74.94mg/g) and ethyl acetate (56.61 mg/g) and juice(23.88 mg/g), where the total flavonoids content (TFC) ended with a result of pomegranate extract with ethanol 35% (18.09 mg/g) higher than the extracted with ethanol 99% (7.95 mg/g) and pomegranate juice (0.0547 mg/g).
HPLC analysis of polyphenolic standards compounds and flavonoids was detected and identified by comparing the peaks retention times in the sample chromatogram of pomegranate peel (extracted using ethanol) with that of the standard. After conducting the HPLC analysis, the results showed, Rutin and Gallic acid
By scavenging free radicals with the DPPH method, the antioxidant activity (AA) of pomegranate peel extracts was identified. Pomegranate peel was extracted using ethanol 99.9% and ethanol 35% as a solvent, yielding results of (51.9 mg/g), (9.86 mg/g), and juice (16.79 mg/g), as opposed to (76.8 mg/g) when ethyl acetate was used as the solvent.
The extracts from peel pomegranate (extracted using ethanol 99.9%) antibacterial activity were determined in vitro using the agar disc diffusion method The existence
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of positive control. the result showed antibacterial activities against Pseudomonas aeruginosa, Escherichia coli, Methicillin-resistant Staphylococcus aureus (MRSA), Staphylococcus aureus and the juice does not have a direct effect on the bacteria, as the bacteria appeared diluted.
The anti-glycation formation of the end product was assessed using an in vitro glucose-bovine serum albumin (BSA) test. The results of the study showed that pomegranate peel extract with ethanol has an anti-glycation effect, whereas pomegranate juice has less of it.
The cream was made based on pomegranate peel extracted using ethanol and had several features, including moisturizing, and smoothing, has a pleasant scent, absorption rapidly (without leaving a film), does not irritate the skin (pH= 5.5-6), has excellent color, easily distributed on the skin
The enzyme HMG-CoA(3-hydroxy-3-methylglutaryl-coenzyme A) reductase is essential for the mevalonate pathway that produces cholesterol. The liver produces less cholesterol when HMG-CoA reductase is present. Pomegranate peel (extracted with 99.9% ethanol) had a 45% inhibitory effect, and pomegranate juice had a 74% inhibitory effect.