Spectroscopic Studies on the Mechanism of Interaction between Vitamin B12 and Vitamin C with Bovine Serum Albumin
dc.contributor.author | Alsamamra, Husain | |
dc.contributor.author | Hawwarin, Ibrahim | |
dc.contributor.author | Abutier, Musa | |
dc.date.accessioned | 2019-12-09T12:20:44Z | |
dc.date.available | 2019-12-09T12:20:44Z | |
dc.date.issued | 2018-07-09 | |
dc.description.abstract | The interaction between vitamin C and vitamin B12 with bovine serum albumin has been investigated. The binding mechanism was studied by UV-absorption and fluorescence spectroscopy. From spectral analysis both vitamins showed strong ability to quench the intrinsic fluorescence of BSA through a static quenching procedure. The binding constants are estimated to be 1.39 x 104 M-1 for vitamin C and 1.61 x 104 M-1 for vitamin B12. FT-IR spectroscopy was used to determine the protein secondary structure. The observed spectral changes indicates an increase of intensity for HSA-vitamin C interaction and a reduction of intensity for HSA-vitamin B12 interaction. While in the difference spectra of vitamin B12-BSA complexes, intensity decreases as the concentration of vitamin B12 increases for amide I. This variation of intensity is related indirectly to the formation of H-bonding in the complex molecules. | en_US |
dc.identifier.citation | TY - JOUR AU - Xu, Hui AU - Liu, Quanwen AU - Zuo, Ying AU - Bi, Yan AU - Gao, Shuli PY - 2009/01/01 SP - 15 EP - 25 T1 - Spectroscopic Studies on the Interaction of Vitamin C with Bovine Serum Albumin VL - 38 DO - 10.1007/s10953-008-9351-6 JO - Journal of Solution Chemistry ER - | en_US |
dc.identifier.issn | 2249 –1929 | |
dc.identifier.uri | https://dspace.alquds.edu/handle/20.500.12213/4999 | |
dc.language.iso | en | en_US |
dc.subject | BSA | en_US |
dc.subject | Vitamin C | en_US |
dc.subject | Vitamin B12 | en_US |
dc.subject | UV-spectroscopy | en_US |
dc.subject | Fluorescence spectroscopy | en_US |
dc.subject | FT-IR | en_US |
dc.title | Spectroscopic Studies on the Mechanism of Interaction between Vitamin B12 and Vitamin C with Bovine Serum Albumin | en_US |
dc.type | Article | en_US |