Spectroscopic Studies on the Mechanism of Interaction between Vitamin B12 and Vitamin C with Bovine Serum Albumin

dc.contributor.authorAlsamamra, Husain
dc.contributor.authorHawwarin, Ibrahim
dc.contributor.authorAbutier, Musa
dc.date.accessioned2019-12-09T12:20:44Z
dc.date.available2019-12-09T12:20:44Z
dc.date.issued2018-07-09
dc.description.abstractThe interaction between vitamin C and vitamin B12 with bovine serum albumin has been investigated. The binding mechanism was studied by UV-absorption and fluorescence spectroscopy. From spectral analysis both vitamins showed strong ability to quench the intrinsic fluorescence of BSA through a static quenching procedure. The binding constants are estimated to be 1.39 x 104 M-1 for vitamin C and 1.61 x 104 M-1 for vitamin B12. FT-IR spectroscopy was used to determine the protein secondary structure. The observed spectral changes indicates an increase of intensity for HSA-vitamin C interaction and a reduction of intensity for HSA-vitamin B12 interaction. While in the difference spectra of vitamin B12-BSA complexes, intensity decreases as the concentration of vitamin B12 increases for amide I. This variation of intensity is related indirectly to the formation of H-bonding in the complex molecules.en_US
dc.identifier.citationTY - JOUR AU - Xu, Hui AU - Liu, Quanwen AU - Zuo, Ying AU - Bi, Yan AU - Gao, Shuli PY - 2009/01/01 SP - 15 EP - 25 T1 - Spectroscopic Studies on the Interaction of Vitamin C with Bovine Serum Albumin VL - 38 DO - 10.1007/s10953-008-9351-6 JO - Journal of Solution Chemistry ER -en_US
dc.identifier.issn2249 –1929
dc.identifier.urihttps://dspace.alquds.edu/handle/20.500.12213/4999
dc.language.isoenen_US
dc.subjectBSAen_US
dc.subjectVitamin Cen_US
dc.subjectVitamin B12en_US
dc.subjectUV-spectroscopyen_US
dc.subjectFluorescence spectroscopyen_US
dc.subjectFT-IRen_US
dc.titleSpectroscopic Studies on the Mechanism of Interaction between Vitamin B12 and Vitamin C with Bovine Serum Albuminen_US
dc.typeArticleen_US
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