Comparison of in situ sequence type analysis of Legionella pneumophila in respiratory tract secretions and environmental samples of a hospital in East Jerusalem

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dc.contributor.authorJaber, Lina
dc.contributor.authorAmro, Mahmod
dc.contributor.authorAbu Tair, Hadeel
dc.contributor.authorBahader, Shereen A.
dc.contributor.authorAlalam, Hanna
dc.contributor.authorButmeh, Suha
dc.contributor.authorAbu Hilal, Dalia
dc.contributor.authorBrettar, Ingrid
dc.contributor.authorHöfle, Manfred G.
dc.contributor.authorBitar, Dina M.
dc.date.accessioned2019-11-17T17:38:26Z
dc.date.available2019-11-17T17:38:26Z
dc.date.issued2018-08-30
dc.description.abstractLegionella pneumophila genotyping is important for epidemiological investigation of nosocomial and community-acquired outbreaks of legionellosis. The prevalence of legionellosis in pneumonia patients in the West Bank was monitored for the first time, and the sequence types (STs) from respiratory samples were compared with STs of environmental samples from different wards of the hospital. Sputum (n = 121) and bronchoalveolar lavage (BAL) (n = 74) specimens were cultured for L. pneumophila; genomic DNA was tested by 16S rRNA polymerase chain reaction (PCR) amplification. Nested PCR sequence-based typing (NPSBT) was implemented on DNA of the respiratory and environmental PCR-positive samples. Only one respiratory specimen was positive for L. pneumophila by culture. BAL gave a higher percentage of L. pneumophila-positive samples, 35% (26/74) than sputum, 15% (18/ 121) by PCR. NPSBT revealed the following STs: ST 1 (29%, 7/24), ST 461 (21%, 5/24), ST 1037 (4%, 1/24) from respiratory samples, STs from environmental samples: ST 1 (28.5%, 4/14), ST 187 (21.4%, 3/14) and ST 2070, ST 461, ST 1482 (7.1%, 1/14) each. This study emphasises the advantage of PCR over culture for the detection of L. pneumophila in countries where antibiotics are indiscriminately used prior to hospital admission. ST 1 was the predominant ST in both respiratory and environmental samples.en_US
dc.description.sponsorshipThis study was supported by a grant from the German Research Foundation (Deutsche Forschungsgemeinschaft DFG) grant number GZ: HO 930/5-1/2. The authors acknowledge Dr Amro Astal and the team in the hospital in Jerusalem for their cooperation in the collection of the clinical samples.en_US
dc.identifier.citationTY - JOUR AU - Jaber, Lina AU - Amro, Mahmod AU - Tair, Hadeel AU - Bahader, Shereen AU - Alalam, Hanna AU - Butmeh, Suha AU - Hilal, Dalia AU - Brettar, Ingrid AU - Höfle, Manfred AU - Bitar, Dina PY - 2018/08/30 SP - 1 EP - 6 T1 - Comparison of in situ sequence type analysis of Legionella pneumophila in respiratory tract secretions and environmental samples of a hospital in East Jerusalem VL - 146 DO - 10.1017/S0950268818002340 JO - Epidemiology and Infection ER -en_US
dc.identifier.issn1469-4409
dc.identifier.urihttps://dspace.alquds.edu/handle/20.500.12213/4918
dc.language.isoenen_US
dc.publisherCambridge University Pressen_US
dc.titleComparison of in situ sequence type analysis of Legionella pneumophila in respiratory tract secretions and environmental samples of a hospital in East Jerusalemen_US
dc.typeArticleen_US
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