Inhibitory Effect of Moringa Oleifera leaf Extract and Olive leaf Extract on Advanced Glycation End Product Formation and Tablet Formulation Development of Moringa Oleifera leaf Extract
Iba' Naser Al Din Ali Halabieh
إباء ناصر الدين علي حلبية
In traditional Palestinian medicine, Olive tree is known for its therapeutic effectiveness against many diseases like diabetes. Where Moringa oleifera is known for the same effectiveness in India. Under hyperglycemic conditions characteristic of type 2 diabetes mellitus, non-enzymatic formation of advanced glycation end products (AGEs) is accelerated and contributes to the development of many complications such as coronary heart disease, stroke, nephropathy, neuropathy, retinopathy and atherosclerosis. Therefore, inhibition of AGE formation represents a potential therapeutic target for the prevention and treatment of diabetic complications. Therefore, the main aim of this study is to investigate the antiglycation effect of each Moringa oleifera and olive leaves extract, and investigating the antiglycation effect of the combination of them by (1:1) ratio. Also to investigate antioxidant activity, total phenolic and total flavonoids content for each of them, and analysing Moringa oleifera leaves extract using liquid chromatograghy HPLC, Moreover, to develop a food supplement from Moringa oleifera leaves extract to be used for diabetic complications. In the present study, 99% ethanolic extracts of Olive leave and Moringa oleifera leave were extracted using Soxhlet apparatus. In vitro glucose-bovine serum albumin (BSA) assay was used to evaluate Anti-glycation formation of end products. Where scavenging effect of the both extracts and the combination of them by (1:1) were evaluated by DPPH assay. Total phenolic and total flavonoids content were estimating by Folin-Ciocalteau assay and colorimetric assay, respectively. HPLC analysis of Moringa oleifera leaves were characterized using Waters HPLC connected to UV- iv PDA detector, while the extract of Moringa oleifera leaves was formulated in a tablet dosage form. The obtained results show that Concentrations of 2.5-12.5 mg/mL of Moringa oleifera leaf extract and Olive leaf extract could inhibit AGE-formation by 13-47% and 0.1% to 36%, respectively. This superior inhibition due to the presence of bioactive compounds in both extracts which reflected in the results of total phenolic content which were 66.5 mg CEQ/g dry extract of Moringa oleifera leaves extrcact and 66.7 mg CEQ/g dry extract of Olive extract and the results of total flavonoids content assays which were 19.04 mg GAE/g dry extract of Moringa oleifera leaves extrcact and 45.2 mg GAE/g dry extract of Olive leaves extract. It was observed that the results for each extract alone were better than combined them together by (1:1) ratio. HPLC chromatogram confirms the presence of bioactive compounds in Moringa oleifera leaves extrcact with identification of Gallic Acid. Whereas tablet formulation indicated that the physical properties of Moringa oleifera leaf extract tablets could be highly enhanced when olive leaf extract added as a binder and active material to the formulation. In the light of the findings of the study it recommends that Moringa oleifera leaf extract and olive leaf extract could be used as a treatment of diabetic complication and promoting over all body health.