Bartonella Species in Fleas from Palestinian Territories: Prevalence and Genetic Diversity

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dc.contributor.authorNasereddin, A.
dc.contributor.authorRisheq, A.
dc.contributor.authorHarrus, S.
dc.contributor.authorAzmi, K.
dc.contributor.authorBaneth, G.
dc.contributor.authorSalant, H.
dc.contributor.authorMumcuoglu, K.Y.
dc.contributor.authorAbdeen, Z.
dc.date.accessioned2018-09-29T13:32:41Z
dc.date.available2018-09-29T13:32:41Z
dc.date.issued2014-11-25
dc.description.abstractBartonellosis is an infectious bacterial disease. The prevalence and genetic characteristics of Bartonella spp. in fleas of wild and domestic animals from Palestinian territories are described. Flea samples (n=289) were collected from 121 cats, 135 dogs, 26 hyraxes and seven rats from northern (n=165), central (n=113), and southern Palestinian territories (n=11). The prevalent flea species were: Ctenocephalides felis (n=119/289; 41.2%), Ctenocephalides canis (n=159/289; 55%), and Xenopsylla sp. (n=7/289; 2.4%). Targeting the Intergenic Transcribed Spacer (ITS) locus, DNA of Bartonella was detected in 22% (64/289) of all fleas. Fifty percent of the C. felis and 57% of the Xenopsylla sp. contained Bartonella DNA. DNA sequencing showed the presence of Bartonella clarridgeiae (50%), Bartonella henselae (27%), and Bartonella koehlerae (3%) in C. felis. Xenopsylla sp. collected from Rattus rattus rats were infected with Bartonella tribocorum, Bartonella elizabethae, and Bartonella rochalimae. Phylogenetic sequence analysis using the 16S ribosomal RNA gene obtained four genetic clusters, B. henselae and B. koehlerae as subcluster 1, B. clarridgeiae as cluster 2, while the rat Bartonella species (B. tribocorum and B. elizabethae) were an outgroup cluster. These findings showed the important role of cat and rat fleas as vectors of zoonotic Bartonella species in Palestinian territories. It is hoped that this publication will raise awareness among physicians, veterinarians, and other health workers of the high prevalence of Bartonella spp. in fleas in Palestinian territories and the potential risk of these pathogens to humans and animals in this region.en_US
dc.description.sponsorshipThis study was a partial fulfillment of MSc degree in the biochemistry and molecular biology program for A. Risheq at Al-Quds University. The study was funded by The Ministry of Foreign Affairs, The Hague, The Netherlands, project M27- 072NVHU 2009 02 ‘Vector-Borne Pathogens in Israel and the Palestinian Authority’.en_US
dc.identifier.issn1948-7134
dc.identifier.urihttps://dspace.alquds.edu/handle/20.500.12213/1022
dc.language.isoen_USen_US
dc.publisherWiley online libraryen_US
dc.subjectBartonella henselaeen_US
dc.subjectBartonella clarridgeiaeen_US
dc.subjectBartonella koehleraeen_US
dc.subjectCtenocephalides felisen_US
dc.subjectIntergenic Transcribed Spaceren_US
dc.subjectPalestinian territoriesen_US
dc.titleBartonella Species in Fleas from Palestinian Territories: Prevalence and Genetic Diversityen_US
dc.typeArticleen_US
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