Formulation and Applications of Borage (Borago Officinalis) seeds oil and leaves extracts and Microemulsion

Marwa Nour Abd AlKarim Garajah
مروه نور عبد الكريم قرجه
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Al-Quds University
The herbal medicine utilizing as one element of complementary and alternative medicine is increasing worldwide, this study aims to prepare a topical borage oil microemulsion formulation to investigate its efficacy and tolerability in the treatment of patients with Atopic Dermatitis. Borage specifically selected in this study due to its abundance in the Palestine Mountains, reachable, the major botanical source of gamma-linolenic acid. The seeds were cultivated upon their ripening season in April of 2016 from the Halhul Mountains. Soxhlet method was used to extract borage seeds and leaves oil by using ethanol 95%. A ternary phase diagram was constructed by determining appropriate nonionic surfactant to assess the ability for microemulsion formulation and durability of each system. Tween 80 was more suitable to solubilize each of borage seeds and leaves extracts compared with Tween 20 due to its prominent hydrophobic properties. The antibacterial activity was evaluated in borage seeds and leaves extracts using a well diffusion technique against Streptococcus aureus, Escherichia coli, Candida albicans. The seeds extracts showed an inhibition zone against S. aureus with 12mm higher than inhibition zone that leaves extracts exhibited against gram-positive bacteria (S. aureus) which reach 7.5mm, but no significant effects for both extracts were reported against E. coli and C. Albicans. In addition the antibacterial activity for microemulsions formulation was measured against each of S. aureus, E. coli, and C. Albicans. As expected, they showed minor positive vi influence against S. aureus when compared to Penicillin G. which is used as a positive control, in contrast no activity was reported against E. coli and C. albicans. The antioxidant activity of borage seeds and leaves extract was investigated by Ferric iron- reducing antioxidant power (FRAP) method, the antioxidant activity was further indicated by the quiet good ability to reduce the FRAP reagents for both extracts with the indication of higher seeds extract activity. This variation is explained by the higher seeds extract content of polyphenol, tocopherol and Vitamin C than leaves extract the content. In results, we can recommend administrating the borage seeds and leaves extracts within the treatment of patients with Atopic Dermatitis, at least in mild cases who are seeking an alternative treatment, in addition to the potential of using it as an effective ingredient within antibacterial and antioxidant applications.