Study the Interaction between Gold Nanoparticles and Bovine Serum Albumin: Spectroscopic Approach

Date
2018-02-28
Authors
Alsamamra, Husain
Hawwarin, Ibrahim
Abu-Sharkh, Sawsan
Abuteir, Musa
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Abstract
Interaction between gold nanoparticles of 40 & 60 nm with BSA has been investigated using UV-absorption spectrophotometry, fluorescence spectroscopy and FT-IR spectroscopy. UV-absorption spectrophotometry showed an increase in the absorption intensity with increasing the molecular ratios of gold nanoparticles of both sizes to BSA, it is found that the value of the binding constant is estimated to be 0.888 × 104 M-1 for gold nanoparticles (40 nm)- BSA complexes, and 1.16 × 104 M-1 for gold nanoparticles (60 nm)-BSA complexes. FT-IR spectroscopy analysis showed that intensities of gold nanoparticles of both sizes-BSA complexes decrease as the concentration increased.
Description
Keywords
Protein, Gold nanoparticles, UV, Fluorescence, FT-IR
Citation
Alsamamra H, Hawwarin I, Abu-Sharkh S, Abuteir M (2018) Study the Interaction between Gold Nanoparticles and Bovine Serum Albumin: Spectroscopic Approach. J Bioanal Biomed 10: 43-49. doi:10.4172/1948-593X.1000203