Comparative studies on the interactions between human serum albumin, bovine serum albumin and cholesterol: ftir and fluorescence spectroscopy
| dc.contributor.author | S., Abu Sharkh | |
| dc.contributor.author | H., Alsamamra | |
| dc.contributor.author | S.M., Darwish | |
| dc.contributor.author | M. M., Abu Teir | |
| dc.date.accessioned | 2019-12-09T12:11:04Z | |
| dc.date.available | 2019-12-09T12:11:04Z | |
| dc.date.issued | 2018-07-30 | |
| dc.description.abstract | The interaction of the human serum albumin (HSA), bovine serum albumin (BSA) with cholesterol has been investigated. The basic binding interaction was studied by FTIR and fluorescence spectroscopy. From spectral analysis cholesterol showed a strong ability to quench the intrinsic fluorescence of HSA and BSA through a static quenching mechanism. The binding constant (k) between HSA and cholesterol is estimated to be K=2.14 × 103 M-1 at 293 K while between BSA and cholesterol is estimated to be K=.1.12 × 103 M-1 at the same temperature. FTIR spectroscopy with Fourier self-deconvolution technique was used to determine the protein secondary structure and cholesterol binding mechanisms. The observed spectral changes indicate a higher percentage of H-bonding between cholesterol and -helix compared to the percentage of H-bonding to cholesterol and -sheets. | en_US |
| dc.description.sponsorship | This work is supported by the German Research Foundation DFG grant No. DR228/24-2 | en_US |
| dc.identifier.citation | Asian Journal of Science and Technology | en_US |
| dc.identifier.issn | 0976-3376 | |
| dc.identifier.uri | https://dspace.alquds.edu/handle/20.500.12213/4998 | |
| dc.language.iso | en | en_US |
| dc.subject | BSA | en_US |
| dc.subject | HSA | en_US |
| dc.subject | binding constant | en_US |
| dc.subject | IR and Fluorescence Spectroscopy | en_US |
| dc.title | Comparative studies on the interactions between human serum albumin, bovine serum albumin and cholesterol: ftir and fluorescence spectroscopy | en_US |
| dc.type | Article | en_US |