Population Genetics of Phlebotomus papatasi from Endemic and Nonendemic Areas for Zoonotic Cutaneous Leishmaniasis in Morocco, as Revealed by Cytochrome Oxidase Gene Subunit I Sequencing

dc.contributor.author Guernaoui, Souad
dc.contributor.author Hamarsheh, Omar
dc.contributor.author Garcia, Deborah
dc.contributor.author Fontenille, Didier
dc.contributor.author Sereno, Denis
dc.date.accessioned 2020-07-21T10:58:01Z
dc.date.available 2020-07-21T10:58:01Z
dc.date.issued 2020-07-06
dc.description.abstract Zoonotic cutaneous leishmaniasis (ZCL) caused by Leishmania major Yakimo & Shokhor and transmitted by Phlebotomus papatasi (Scopoli) is a public health concern in Morocco. The disease is endemic mainly in pre-Saharan regions on the southern slope of the High Atlas Mountains. The northern slope of the High Atlas Mountains and the arid plains of central Morocco remain non-endemic and are currently considered high risk for ZCL. Here we investigate and compare the population genetic structure of P. papatasi populations sampled in various habitats in historical foci and non-endemic ZCL areas. A fragment of the mtDNA cytochrome oxidase I (COI) gene was amplified and sequenced in 59 individuals from 10 P. papatasi populations. Haplotype diversity was probed, a median-joining network was generated (FST) and molecular variance (AMOVA) were analyzed. Overall, we identified 28 haplotypes with 32 distinct segregating sites, of which seven are parsimony informative. The rate of private haplotypes was high; 20 haplotypes (71.4%) are private ones and exclusive to a single population. The phylogenetic tree and the network reconstructed highlight a genetic structuration of these populations in two well defined groups: Ouarzazate (or endemic areas) and Non-Ouarzazate (or nonendemic areas). These groups are separated by the High Atlas Mountains. Overall, our study highlights di erences in terms of population genetics between ZCL endemic and non-endemic areas. To what extent such di erences would impact the transmission of L. major by natural P. papatasi population remains to be investigated. en_US
dc.description.sponsorship Funding: This research received no external funding. Acknowledgments: We thank the anonymous reviewers for their helpful suggestions, corrections and comments on a previous draft of the manuscript. en_US
dc.identifier.issn 2076-2607
dc.identifier.uri https://dspace.alquds.edu/handle/20.500.12213/5708
dc.language.iso en en_US
dc.publisher MDPI en_US
dc.subject Phlebotomus papatasi en_US
dc.subject Leishmania major en_US
dc.subject zoonotic cutaneous leishmaniasis en_US
dc.subject genetic structure en_US
dc.subject Cytochrome oxidase subunit I en_US
dc.subject Morocco en_US
dc.title Population Genetics of Phlebotomus papatasi from Endemic and Nonendemic Areas for Zoonotic Cutaneous Leishmaniasis in Morocco, as Revealed by Cytochrome Oxidase Gene Subunit I Sequencing en_US
dc.type Article en_US
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