Oleuropein Is Responsible for the Major Anti-Inflammatory Effects of Olive Leaf Extract
| dc.contributor.author | Qabaha, Khaled | |
| dc.contributor.author | AL-Rimawi, Fuad | |
| dc.contributor.author | Qasem, Ahmad | |
| dc.contributor.author | Naser, Saleh A. | |
| dc.date.accessioned | 2020-02-23T19:51:21Z | |
| dc.date.available | 2020-02-23T19:51:21Z | |
| dc.date.issued | 2017-08-17 | |
| dc.description.abstract | Olive leaves are rich in polyphenolic compounds that are known to have antioxidant, antimicrobial, and antiinflammatory activities. Therefore, olive leaf extract (OLE) is considered as a natural supplement. In this study we evaluated the antibacterial and the anti-inflammatory effect of OLE and its individual phenolic components in vitro. Polymorphonuclear cells (PMNCs) were isolated from the whole blood using Histopaque solution and cultured in RPMI-enriched medium. Tumor necrosis factor a (TNFa) level was determined by ELISA after 24 h of lipopolysaccharide stimulation. The antibacterial activity of OLE was determined by well diffusion assay. We found a significant decrease in TNFa secretion level in PMNCs culture treated with OLE. Oleuropein is the only OLE component that has shown anti-inflammatory effects at a concentration of 20 lg/mL. Furthermore, OLE exhibited antibacterial activity against some gram positive bacterial strains; however, gram negative bacterial strains were resistant to OLE. Downregulation of TNFa secretion in PMNCs culture in response to OLE treatment indicates that this polyphenol-rich extract has an anti-inflammatory effect, and oleuropein is the major OLE component responsible for this effect. The antibacterial activity of OLE is limited to gram positive bacteria. | en_US |
| dc.description.sponsorship | Our thanks are due to all laboratory members who participated in this study. | en_US |
| dc.identifier.citation | TY - JOUR AU - Qabaha, Khaled AU - Al-Rimawi, Fuad AU - Qasem, Ahmad AU - Naser, Saleh PY - 2017/11/03 SP - T1 - Oleuropein Is Responsible for the Major Anti-Inflammatory Effects of Olive Leaf Extract VL - 21 DO - 10.1089/jmf.2017.0070 JO - Journal of Medicinal Food ER - | en_US |
| dc.identifier.issn | 1096-620X | |
| dc.identifier.uri | https://dspace.alquds.edu/handle/20.500.12213/5076 | |
| dc.language.iso | en | en_US |
| dc.subject | gallic acid | en_US |
| dc.subject | OLE | en_US |
| dc.subject | oleuropein | en_US |
| dc.subject | olive leaf extract | en_US |
| dc.subject | phenolic compounds | en_US |
| dc.subject | TNFa | en_US |
| dc.title | Oleuropein Is Responsible for the Major Anti-Inflammatory Effects of Olive Leaf Extract | en_US |
| dc.type | Article | en_US |