Complete genome sequencing of SARS-CoV-2 strains: A pilot survey in Palestine reveals spike mutation H245N
| dc.contributor.author | Al-Jawabreh A. | |
| dc.contributor.author | Ereqat S. | |
| dc.contributor.author | Dumaidi K. | |
| dc.contributor.author | Al-Jawabreh H. | |
| dc.contributor.author | Nasereddin A. | |
| dc.date.accessioned | 2024-11-26T07:47:14Z | |
| dc.date.available | 2024-11-26T07:47:14Z | |
| dc.date.issued | 2021-12-23 | |
| dc.description.abstract | Objectives: SARS-CoV-2, severe respiratory syndrome coronavirus-2, is an RNA virus that emerged from China sweeping the globe in the form of a pandemic that became an international public health concern. This pilot study aimed to describe the genetic variation and molecular epidemiology of SARS-CoV-2 in Palestine in fall 2020. Results: To achieve these aims, whole genome sequencing of SARS-CoV-2, phylogenetic analysis, haplotype networking and genetic diversity analysis were performed. These analyses revealed a unique spike mutation H245N that has never been reported before. The phylogenetic analysis depicted that three clusters existed in Palestinian SARS-CoV-2 genome sequences, in which cluster-I comprised the majority of clusters by 90%. Congruently, the haplotype network analysis depicted the same three clusters with a total of 39 haplotypes. The genetic diversity analysis showed that Cluster-I is highly diverse as confirmed by statistically significant mutation rate indices, Tajima's D and Fu-Li's-F tests (- 2.11 and 2.74, respectively), highest number of mutations (Eta = 120), highest number of haplotypes (h = 17), and highest average number of nucleotide differences between any two sequences (S = 118). The study confirmed the high genetic diversity among the Palestinian of SARS-CoV-2 which possessed high number of mutations including one which was reported for the first time. | |
| dc.identifier.uri | https://dspace.alquds.edu/handle/20.500.12213/9496 | |
| dc.title | Complete genome sequencing of SARS-CoV-2 strains: A pilot survey in Palestine reveals spike mutation H245N | |
| dc.type | Animation |