Moroccan Leishmania infantum: Genetic Diversity and Population Structure as Revealed by Multi-Locus Microsatellite Typing

dc.contributor.author Amro, Ahmad
dc.contributor.author Hamdi, Salsabil
dc.contributor.author Lemrani, Meryem
dc.contributor.author Mouna, Idrissi
dc.contributor.author Mohammed, Hida
dc.contributor.author Mostafa, Sabri
dc.contributor.author Rhajaoui, Mohamed
dc.contributor.author Hamarsheh, Omar
dc.date.accessioned 2018-09-03T10:45:05Z
dc.date.available 2018-09-03T10:45:05Z
dc.date.issued 2013-10-17
dc.description.abstract Leishmania infantum causes Visceral and cutaneous leishmaniasis in northern Morocco. It predominantly affects children under 5 years with incidence of 150 cases/year. Genetic variability and population structure have been investigated for 33 strains isolated from infected dogs and humans in Morocco. A multilocus microsatellite typing (MLMT) approach was used in which a MLMtype based on size variation in 14 independent microsatellite markers was compiled for each strain. MLMT profiles of 10 Tunisian, 10 Algerian and 21 European strains which belonged to zymodeme MON-1 and non-MON-1 according to multilocus enzyme electrophoresis (MLEE) were included for comparison. A Bayesian model-based approach and phylogenetic analysis inferred two L.infantum sub-populations; Sub-population A consists of 13 Moroccan strains grouped with all European strains of MON-1 type; and subpopulation B consists of 15 Moroccan strains grouped with the Tunisian and Algerian MON-1 strains. Theses subpopulations were significantly different from each other and from the Tunisian, Algerian and European non MON-1 strains which constructed one separate population. The presence of these two sub-populations co-existing in Moroccan endemics suggests multiple introduction of L. infantum from/to Morocco; (1) Introduction from/to the neighboring North African countries, (2) Introduction from/to the Europe. These scenarios are supported by the presence of sub-population B and sub-population A respectively. Gene flow was noticed between sub-populations A and B. Five strains showed mixed A/B genotypes indicating possible recombination between the two populations. MLMT has proven to be a powerful tool for eco-epidemiological and population genetic investigations of Leishmania. en_US
dc.description.sponsorship The fieldwork and data collection was granted by Pastur Institute in Morocco for Dr. Meryem Lemrani. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. en_US
dc.identifier.citation Amro A, Hamdi S, Lemrani M, Mouna I, Mohammed H, et al. (2013) Moroccan Leishmania infantum: Genetic Diversity and Population Structure as Revealed by Multi-Locus Microsatellite Typing. PLoS ONE 8(10): e77778. doi:10.1371/journal.pone.0077778 en_US
dc.identifier.issn 1932-6203
dc.identifier.uri https://dspace.alquds.edu/handle/20.500.12213/823
dc.language.iso en_US en_US
dc.publisher Public Library of Science en_US
dc.title Moroccan Leishmania infantum: Genetic Diversity and Population Structure as Revealed by Multi-Locus Microsatellite Typing en_US
dc.type Article en_US
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