Development and Validation of RP-HPLC Method for the Simultaneous Determination of Trimethoprim, Sulfadimidine Sodiumand Tylosin Tartrate in injectable solution formulation

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dc.contributor.authorGhanem, Mashhour
dc.contributor.authorAbu-Lafi, Saleh
dc.date.accessioned2018-09-22T09:01:22Z
dc.date.available2018-09-22T09:01:22Z
dc.date.issued2015-01-03
dc.description.abstractA simple, robust and reliable reversed phase HPLC method was developed and validated for the simultaneous determination of Trimethoprim (TMP), Sulfadimidine sodium (SDMS) and Tylosin tartrate (TYT) in Nuroprim® injectable solution formulation. The desired separation was achieved on XBridge C18 column (150  4.6 mm i.d., 5m) at room temperature. The optimized mobile phase consisted of a binary solvent mixture of acetonitrile and aqueous triethylamine (TEA) solution adjusted to pH 5.7 by acetic acid. The mobile phase flow rate was fixed at 1.0 ml/min and the analytes were monitored at 287 nm using photodiode array detector. The effects of the chromatographic conditions on peaks capacity factor, USP tailing factor, column efficiency and resolution were systematically optimized. The method was validated as per International Conference of Harmonization (ICH) and United States Pharmacopeia (USP) guidelines and found to be adequate for the routine quantitative determination of TMP, SDMS and TYT in commercially available Nuroprim® injectable solution dosage form.en_US
dc.description.sponsorshipWe would like to thanks Pharmacare pharmaceutical company (Palestine) for their continuous support.en_US
dc.identifier.citationMashhour Ghanem, Saleh Abu-Lafi. Development and Validation of RP-HPLC Method for the Simultaneous Determination of Trimethoprim, Sulfadimidine Sodium and Tylosin Tartrate in injectable solution formulation. J App Pharm Sci, 2015; 5 (01): 094-098.en_US
dc.identifier.issn2231-3354
dc.identifier.urihttps://dspace.alquds.edu/handle/20.500.12213/963
dc.language.isoen_USen_US
dc.subjectTylosin Tartrateen_US
dc.subjectSulfadimidine sodiumen_US
dc.subjectTrimethoprimen_US
dc.subjectHPLCen_US
dc.subjectInjectable solutionen_US
dc.titleDevelopment and Validation of RP-HPLC Method for the Simultaneous Determination of Trimethoprim, Sulfadimidine Sodiumand Tylosin Tartrate in injectable solution formulationen_US
dc.typeArticleen_US
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