Spectroscopic Characterization of the Interaction between Dopamine and Human Serum Albumin

dc.contributor.author Khalid, Imtiaz M.
dc.contributor.author Abu Sharkh, Sawsan E.
dc.contributor.author Samamarh, Husain
dc.contributor.author Alfaqeeh, Rania
dc.contributor.author Abuteir, Musa M.
dc.contributor.author Darwish, Saqer M.
dc.date.accessioned 2019-03-03T09:19:59Z
dc.date.available 2019-03-03T09:19:59Z
dc.date.issued 2019-02-27
dc.description.abstract The interactions of HSA with DA have received great attention nowadays due to its significant effect in the biomedical field and overall health. The main aim of this work is to examine the interaction between DA and HSA at physiological conditions. Upon addition of DA to HSA, the fluorescence emission was quenched with quenching constant Kq = 1.32 × 109 L∙mol−1∙s−1 and the binding constant of DA with HSA is found to be K = 4.4 × 102 mainly indicating dynamic quenching. The HSA conformation was altered upon binding of DA to HSA with an increase in α-helix and a decrease in β-sheets suggesting unfolding of HSA secondary structure due to weak intermolecular interaction with HSA. In view of the evidence presented, it is important to understand the details of the interactions of HSA with DA which will be crucial in the design of new DA-inspired drugs and help revealing vital details to better understand the HSA’s role as a transporter for many drugs. en_US
dc.description.sponsorship This work is supported by the German Research Foundation DFG Grant No. DR228/24-2. en_US
dc.identifier.citation Khalid, I.M., Abu Sharkh, S.E., Samamarh, H., Alfaqeeh, R., Abuteir, M.M. and Darwish, S.M. (2019) Spectroscopic Characterization of the Interaction between Dopamine and Human Serum Albumin. Open Journal of Biophysics , 9, 110-130. https://doi.org/10.4236/ojbiphy.2019.92009 en_US
dc.identifier.issn 2164-5396
dc.identifier.uri https://dspace.alquds.edu/handle/20.500.12213/4743
dc.language.iso en en_US
dc.subject Dopamine en_US
dc.subject HSA en_US
dc.subject Binding Constant en_US
dc.subject Non-Linear Stern-Volmer Plot en_US
dc.subject Protein Secondary Structure en_US
dc.subject FT-IR Spectroscopy en_US
dc.title Spectroscopic Characterization of the Interaction between Dopamine and Human Serum Albumin en_US
dc.type Article en_US
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
4-1850185.pdf
Size:
1.5 MB
Format:
Adobe Portable Document Format
Description:
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description: