Spectroscopic Characterization of the Interaction between Dopamine and Human Serum Albumin

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dc.contributor.authorKhalid, Imtiaz M.
dc.contributor.authorAbu Sharkh, Sawsan E.
dc.contributor.authorSamamarh, Husain
dc.contributor.authorAlfaqeeh, Rania
dc.contributor.authorAbuteir, Musa M.
dc.contributor.authorDarwish, Saqer M.
dc.date.accessioned2019-03-03T09:19:59Z
dc.date.available2019-03-03T09:19:59Z
dc.date.issued2019-02-27
dc.description.abstractThe interactions of HSA with DA have received great attention nowadays due to its significant effect in the biomedical field and overall health. The main aim of this work is to examine the interaction between DA and HSA at physiological conditions. Upon addition of DA to HSA, the fluorescence emission was quenched with quenching constant Kq = 1.32 × 109 L∙mol−1∙s−1 and the binding constant of DA with HSA is found to be K = 4.4 × 102 mainly indicating dynamic quenching. The HSA conformation was altered upon binding of DA to HSA with an increase in α-helix and a decrease in β-sheets suggesting unfolding of HSA secondary structure due to weak intermolecular interaction with HSA. In view of the evidence presented, it is important to understand the details of the interactions of HSA with DA which will be crucial in the design of new DA-inspired drugs and help revealing vital details to better understand the HSA’s role as a transporter for many drugs.en_US
dc.description.sponsorshipThis work is supported by the German Research Foundation DFG Grant No. DR228/24-2.en_US
dc.identifier.citationKhalid, I.M., Abu Sharkh, S.E., Samamarh, H., Alfaqeeh, R., Abuteir, M.M. and Darwish, S.M. (2019) Spectroscopic Characterization of the Interaction between Dopamine and Human Serum Albumin. Open Journal of Biophysics , 9, 110-130. https://doi.org/10.4236/ojbiphy.2019.92009en_US
dc.identifier.issn2164-5396
dc.identifier.urihttps://dspace.alquds.edu/handle/20.500.12213/4743
dc.language.isoenen_US
dc.subjectDopamineen_US
dc.subjectHSAen_US
dc.subjectBinding Constanten_US
dc.subjectNon-Linear Stern-Volmer Ploten_US
dc.subjectProtein Secondary Structureen_US
dc.subjectFT-IR Spectroscopyen_US
dc.titleSpectroscopic Characterization of the Interaction between Dopamine and Human Serum Albuminen_US
dc.typeArticleen_US
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