Concurrent molecular characterization of sand fies and Leishmania parasites by amplicon-based next-generation sequencing

Date
2022-07-22
Authors
Abedelmajeed Nasereddin
Suheir Ereqat
Amer Al‐Jawabreh
Mohamad Taradeh
Ibrahim Abbasi
Hanan Al‐Jawabreh
Samer Sawalha
Ziad Abdeen
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Publisher
BMC
Abstract
Phlebotomine sand flies are vectors of Leishmania parasites, which are the causative agents of leishmaniasis. Herein, we developed an amplicon-based next-generation sequencing (Amp-NGS) to characterize sand flies and Leishmania parasites simultaneously targeting partial fragments of 18S rDNA and ITS1 genes, respectively.
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