MiR-16-1* and miR-16-2* possess strong tumor suppressive and anti-metastatic properties in osteosarcoma

Simple item page
dc.contributor.authorMaximov, Vadim V.
dc.contributor.authorKhawaled, Saleh
dc.contributor.authorSalah, Zaidoun
dc.contributor.authorJaber, Lina
dc.contributor.authorBengaiev, Nataly
dc.contributor.authorBarhoum, Ahlam
dc.contributor.authorGalasso, Marco
dc.contributor.authorYavin, Eylon
dc.contributor.authorAqeilan, Rami I.
dc.date.accessioned2019-11-22T08:28:26Z
dc.date.available2019-11-22T08:28:26Z
dc.date.issued2018-09-09
dc.description.abstractOsteosarcoma (OS) is an aggressive malignancy affecting mostly children and adolescents. MicroRNAs (miRNAs) play important roles in OS development and progression. Here we found that miR-16-1* and miR-16-2* “passenger” strands as well as the “lead” miR-16 strand possess strong tumor suppressive functions in human OS. We report different although strongly overlapping functions for miR-16-1* and miR-16-2* in OS cells. Ectopic expression of these miRNAs affected primary tumor growth, metastasis seeding, and chemoresistance and invasiveness of human OS cells. Loss-of-function experiments verified tumor suppressive functions of these miRNAs at endogenous levels of expression. Using RNA immunoprecipitation (RIP) assays, we identify direct targets of miR-16-1* and miR-16-2* in OS cells. Furthermore, validation experiments identified FGFR2 as a direct target for miR-16-1* and miR-16-2*. Overall, our findings underscore the importance of passenger strand miRNAs in osteosarcomagenesis.en_US
dc.identifier.citationTY - BOOK AU - Maximov, Vadim AU - Khawaled, Saleh AU - Salah, Zaidoun AU - Jaber, Lina AU - Bengaiev, Nataly AU - Barhoum, Ahlam AU - Galasso, Marco AU - Yavin, Eylon AU - Aqeilan, Rami PY - 2018/09/09 SP - T1 - MiR-16-1* and miR-16-2* possess strong tumor suppressive and anti-metastatic properties in osteosarcoma. DO - 10.1101/412411 ER -en_US
dc.identifier.urihttps://dspace.alquds.edu/handle/20.500.12213/4959
dc.language.isoenen_US
dc.titleMiR-16-1* and miR-16-2* possess strong tumor suppressive and anti-metastatic properties in osteosarcomaen_US
dc.typeArticleen_US
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
412411.full.pdf
Size:
7.13 MB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
AQU researchers publications