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dc.contributor.authorAbdul-Ghani, Safa
dc.contributor.authorYanai, Joseph
dc.contributor.authorAbdul-Ghani, Rula
dc.contributor.authorPinkas, Adi
dc.contributor.authorAbdeen, Ziad
dc.date.accessioned2018-09-17T10:35:23Z
dc.date.available2018-09-17T10:35:23Z
dc.date.issued2011-10-13
dc.identifier.issn0892-0362
dc.identifier.urihttps://dspace.alquds.edu/handle/20.500.12213/940
dc.description.abstractPhthalates are industrial chemicals widely used in consumer products, plastics and children toys, and the risk of exposure to phthalates, especially prenatal exposure, is a growing concern justifying the development of an animal model to better understand their effect. The present study was designed to evaluate the suitability of a chick model for phthalate DEHP teratogenicity and neurobehavioral teratogenicity, a model which is simple and devoid of potential confounding factors such as maternal toxicity, maternal-fetal unit and maternalneonatal interactions; major findings were confirmed in the DBP study. Prehatch exposure to DEHP in doses ranging from 20 to 100 mg/kg, reduced the percent hatching from 80% in control eggs to 65%, and increased late hatchings from 12.5% in control eggs to 29.4%. In addition it induced developmental defects characterized by an opening or weakening of abdominal muscles allowing internal organs to protrude externally with or without a sac, omphalocele or gastroschisis, respectively. The effect was dose dependent ranging from 8% with DEHP (20 mg/kg) to 22% (100 mg/kg). Similar treatment with DBP 100 mg/kg has reduced percentage hatching to 57% and increased late hatching to 37.5%, with a 14% increase in gastroschisis. Biochemical evaluation revealed elevated levels of alkaline phosphatase, which reflects non-specific toxicity of DEHP at such a high dose. Behavioral evaluation using an imprinting test and locomotor activity on chicks pretreated with DEHP (100 mg/kg) has shown an abolishment of imprinting performance from the control (0.65) preference ratio. DNA damage measurements of the metabolite 8-hydroxydeoxyguanosine (8-OH-dG) in blood samples showed an increase of 39.7% after prehatch exposure to phthalates. This was statistically significant for DEHP and indicates genetic toxicity, since part of the teratogenic activity is associated with oxidative stress and DNA damage.en_US
dc.description.sponsorshipThis study was supported by a grant from the Al-Quds Nutrition & Health Research Institute. The authors would like to thank Mr. Rateb Hussein and Mr. Munther Metani for their technical assistance, and Dr. Tamer Essawi and Mr. Firas Hassan for their help in performing the biochemical measurements.en_US
dc.language.isoen_USen_US
dc.publisherElsevieren_US
dc.subjectDNA damageen_US
dc.subjectEmbryonic developmenten_US
dc.subjectGastroschisisen_US
dc.subjectNeurobehavioral teratogenicityen_US
dc.subjectOmphaloceleen_US
dc.subjectPhthalatesen_US
dc.titleThe teratogenicity and behavioral teratogenicity of di(2-ethylhexyl) phthalate (DEHP) and di-butyl Phthalate (DBP) in a chick modelen_US
dc.typeArticleen_US


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