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dc.contributor.authorAl-Jawabreh, Amer
dc.contributor.authorDumaidi, Kamal
dc.contributor.authorEreqat, Suheir
dc.contributor.authorNasereddin, Abedelmajeed
dc.contributor.authorAl-Jawabreh, Hanan
dc.contributor.authorAzmi, Kifaya
dc.contributor.authorAl-Laham, Nahed
dc.contributor.authorAbdeen, Ziad
dc.date.accessioned2018-08-26T11:09:11Z
dc.date.available2018-08-26T11:09:11Z
dc.date.issued2015-07-16
dc.identifier.citationAl-Jawabreh A, Dumaidi K, Ereqat S, Nasereddin A, Al-Jawabreh H, Azmi K, et al. (2015) Incidence of Echinococcus granulosus in Domestic Dogs in Palestine as Revealed by Copro-PCR. PLoS Negl Trop Dis 9(7): e0003934. doi:10.1371/journal. pntd.0003934en_US
dc.identifier.urihttps://dspace.alquds.edu/handle/20.500.12213/786
dc.description.abstractHydatidosis or echinococcosisis considered a neglected zoonotic disease despite its high burden in the livestock industry and the high risk of infection by humans in endemic areas. In a cross-sectional study we estimated the copro-Incidence and also genotyped Echinococcus granulosus isolates from domestic dogs using polymerase chain reaction (PCR). Medical archives in nine major hospitals in Palestine were reviewed to determine incidence of E. granulosus infection detected in humans during surgery. Faecal samples were collected from 93 domestic dogs in three districts with the highest number of human cases: Al- Khalil (Hebron), Tubas and Jenin. Genomic DNA was extracted from dog faecal samples and amplified by PCR targeting the repeat DNA sequence (EgG1 Hae III) followed by sequencing of five positive samples. Genotyping was determined by sequencing and BLAST searching of mitochondrial cytochrome c oxidase subunit (CO1). The incidence of E. granulosus infection detected in humans at surgery was 1.2 per 100,000 in the West Bank and 1.0 per 100,000 in Gaza Strip. Seventeen of 93 domestic dogs (18%) were positive, based upon comparison with the Echinococcus DNA control. The five sequenced samples were confirmed to be E. granulosus. Successfully genotyped sample belonged to E. granulosus sensu stricto (formerly G1-G3 complex, sheep strain). For domestic dogs, age group (13-24 months) and sex were identified as two risk factors for contracting E. granulosus. The study identified the high incidence of E. granulosus sensu stricto in dogs in Palestine. Authoren_US
dc.description.sponsorshipWe thank the Arab American University in Jenin-Palestine for the fund received under grant number 2013-104, cycle 2. Also, the study received support from the Netherlands Ministry of Foreign Affairs, The Hague, The Netherlands and NVHU under grant reference number 2014.52146. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.en_US
dc.language.isoen_USen_US
dc.publisherGrossMarken_US
dc.titleIncidence of Echinococcus granulosus in Domestic Dogs in Palestine as Revealed by Copro-PCRen_US
dc.typeArticleen_US


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